Development of transgenic cabbage (Brassica oleracea var. capitata) for insect resistance by agrobacterium tumefaciens-mediated transformation

R. G. Jin, Y. B. Liu, B. E. Tabashnik, D. Borthakur

Research output: Contribution to journalArticle

53 Scopus citations

Abstract

Transgenic head cabbage (Brassica oleracea var. capitata), resistant to diamondback moth (Plutella xylostella) larvae, was developed through Agrobacterium tumefaciens-mediated transformation with Bacillus thuringiensis (Bt) cry genes using a modified procedure. Factors important for transformation included cabbage cultivar; preculture and coculture of explants on a callus initiation medium; use of appropriate amount; and delay in initial application of selective agents. A total of 15 independent transformed lines with over 100 plants were obtained from several transformation experiments, representing an overall transformation efficiency of ~ 1%. Cabbage plants transformed with a synthetic Bt gene, cry1Ab3, were all resistant to larvae of the diamondback moth, whereas all plants transgenic for cry1Ia3, a wild-type Bt gene, were susceptible. As a first step towards testing the hypothesis that reduced exposure of Bt to target insects would delay the evolution of insect resistance to Bt, cry1Ab3 expression was put under the transcriptional control of the soybean wound-inducible vspB promoter and transgenic cabbage was obtained. Insect bioassay showed that such plants were all resistant to diamondback moth even without induction for the expression of Bt.

Original languageEnglish (US)
Pages (from-to)231-237
Number of pages7
JournalIn Vitro Cellular and Developmental Biology - Plant
Volume36
Issue number4
DOIs
StatePublished - Jan 1 2000

Keywords

  • Brassica
  • Diamondback moth
  • Genetic transformation
  • Inducible expression
  • cry genes

ASJC Scopus subject areas

  • Biotechnology
  • Plant Science

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