Developmental expression and comparative genomic analysis of Xenopus cardiac myosin heavy chain genes

Robert J. Garriock, Stryder M. Meadows, Paul A. Krieg

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Myosin heavy chains (MHC) are cytoskeletal motor proteins essential to the process of muscle contraction. We have determined the complete sequences of the Xenopus cardiac MHC genes, α-MHC and ventricular MHC (vMHC), and have characterized their developmental expression profiles. Whereas α-MHC is expressed from the earliest stages of cardiac differentiation, vMHC transcripts are not detected until the heart has undergone chamber formation. Early expression of vMHC appears to mark the cardiac conduction system, but expression expands to include the ventricle and outflow tract myocardium during subsequent development. Sequence comparisons, transgenic expression analysis, and comparative genomic studies indicate that Xenopus α-MHC is the true orthologue of the mammalian α-MHC gene. On the other hand, we show that the Xenopus vMHC gene is most closely related to chicken ventricular MHC (vMHC1) not the mammalian β-MHC. Comparative genomic analysis has allowed the detection of a mammalian MHC gene (MyH15) that appears to be the orthologue of vMHC, but evidence suggests that this gene is no longer active.

Original languageEnglish (US)
Pages (from-to)1287-1293
Number of pages7
JournalDevelopmental Dynamics
Volume233
Issue number4
DOIs
StatePublished - Aug 2005

Keywords

  • MHC-alpha
  • MyH15
  • Synteny
  • aMHC
  • vMHC

ASJC Scopus subject areas

  • Developmental Biology

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