Developmental expression of N-acetyltransferases in C57BI/6 mice

M. Kimberly Mitchell, Bernard W Futscher, Charlene A. McQueen

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

N-Acetyltransferases (NATs) play an important role in the biotransformation of a wide variety of arylamine drugs and carcinogens. Two genes (NAT1, NAT2) have been identified and allelic variation in NAT2 has been associated with arylamine toxicity in adults. Little information has been reported on expression of NAT genes during embryonic and fetal development although substrate specific NAT activity has been detected. The current study investigated the expression of NAT1 and NAT2 in mice pre- and postnatally. RNA was isolated from maternal liver, embryonic tissue at gestational days (GD) 10, 15, and 18, or neonates at neonatal day 3. Reverse transcription-polymerase chain reaction was performed using primers designed to amplify portions of either the NAT1 or the NAT2 gene. NAT1 and NAT2 mRNAs were detected in the embryo/placental complex at GD 10 and in GD 15 and 18 embryos. NAT2 but not NAT1 was expressed in GD 18 and neonatal day 3 hepatic tissue. These data demonstrate the differential expression of NAT genes in the mouse embryo and suggest a potential role for NAT in development.

Original languageEnglish (US)
Pages (from-to)261-264
Number of pages4
JournalDrug Metabolism and Disposition
Volume27
Issue number2
StatePublished - 1999

Fingerprint

Acetyltransferases
Genes
Embryonic Structures
Embryonic and Fetal Development
Tissue
Liver
Polymerase chain reaction
Transcription
Biotransformation
Carcinogens
Reverse Transcription
Toxicity
Mothers
RNA
Polymerase Chain Reaction
Messenger RNA
Substrates
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Pharmacology
  • Toxicology

Cite this

Developmental expression of N-acetyltransferases in C57BI/6 mice. / Mitchell, M. Kimberly; Futscher, Bernard W; McQueen, Charlene A.

In: Drug Metabolism and Disposition, Vol. 27, No. 2, 1999, p. 261-264.

Research output: Contribution to journalArticle

Mitchell, M. Kimberly ; Futscher, Bernard W ; McQueen, Charlene A. / Developmental expression of N-acetyltransferases in C57BI/6 mice. In: Drug Metabolism and Disposition. 1999 ; Vol. 27, No. 2. pp. 261-264.
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