The developmental aspects of calcium transport across the intestinal brush membrane vesicles was studied utilizing a millipore filtration technique. Calcium transport represented uptake into the intravesicular space as evidenced by osmolality studies, calcium release by the calcium ionophore A23187, and temperature dependency. Calcium transport in both suckling and adolescent rats appears to occur by a saturable mechanism. Calcium uptake was similar in the presence of sodium and potassium gradients, but decreased in the presence of choline gradient. The imposition of negative membrane potential did not enhance calcium uptake compared to voltage clamp conditions indicating an electroneutral process. The initial rate of calcium uptake was linear up to 15 s. Kinetic analysis of calcium uptake at 7 s showed lower Vm,% and lower Km in suckling rats compared to adolescent rats. These studies are the first to demonstrate the maturational aspects of calcium entry at the brush border level and are consistent with our previous kinetic studies utilizing whole tissue.
ASJC Scopus subject areas
- Pediatrics, Perinatology, and Child Health