Developmental nicotine exposure enhances inhibitory synaptic transmission in motor neurons and interneurons critical for normal breathing

Stuti J. Jaiswal, Lila Buls Wollman, Caitlyn M. Harrison, Jason Q. Pilarski, Ralph F Fregosi

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Nicotine exposure in utero negatively affects neuronal growth, differentiation, and synaptogenesis. We used rhythmic brainstems slices and immunohistochemistry to determine how developmental nicotine exposure (DNE) alters inhibitory neurotransmission in two regions essential to normal breathing, the hypoglossal motor nucleus (XIIn), and preBötzinger complex (preBötC). We microinjected glycine or muscimol (GABAA agonist) into the XIIn or preBötC of rhythmic brainstem slices from neonatal rats while recording from XII nerve roots to obtain XII motoneuron population activity. Injection of glycine or muscimol into the XIIn reduced XII nerve burst amplitude, while injection into the preBötC altered nerve burst frequency. These responses were exaggerated in preparations from DNE animals. Quantitative immunohistochemistry revealed a significantly higher GABAA receptor density on XII motoneurons from DNE pups. There were no differences in GABAA receptor density in the preBötC, and there were no differences in glycine receptor expression in either region. Nicotine, in the absence of other chemicals in tobacco smoke, alters normal development of brainstem circuits that are critical for normal breathing.

Original languageEnglish (US)
Pages (from-to)337-354
Number of pages18
JournalDevelopmental Neurobiology
Volume76
Issue number3
DOIs
StatePublished - Mar 1 2016

Fingerprint

Motor Neurons
Interneurons
Nicotine
Synaptic Transmission
Respiration
Hypoglossal Nerve
Brain Stem
Muscimol
GABA-A Receptors
Glycine
Immunohistochemistry
GABA-A Receptor Agonists
Glycine Receptors
Injections
Smoke
Tobacco
Growth
Population

Keywords

  • Brainstem slice
  • GABA
  • Glycine
  • Hypoglossal motoneurons
  • PreBötzinger complex

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Developmental Neuroscience

Cite this

Developmental nicotine exposure enhances inhibitory synaptic transmission in motor neurons and interneurons critical for normal breathing. / Jaiswal, Stuti J.; Buls Wollman, Lila; Harrison, Caitlyn M.; Pilarski, Jason Q.; Fregosi, Ralph F.

In: Developmental Neurobiology, Vol. 76, No. 3, 01.03.2016, p. 337-354.

Research output: Contribution to journalArticle

Jaiswal, Stuti J. ; Buls Wollman, Lila ; Harrison, Caitlyn M. ; Pilarski, Jason Q. ; Fregosi, Ralph F. / Developmental nicotine exposure enhances inhibitory synaptic transmission in motor neurons and interneurons critical for normal breathing. In: Developmental Neurobiology. 2016 ; Vol. 76, No. 3. pp. 337-354.
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