Developmental regulation of a gastrula-specific gene injected into fertilized Xenopus eggs.

P. A. Krieg, D. A. Melton

Research output: Contribution to journalArticle

114 Scopus citations

Abstract

To study the transcriptional regulation of genes during early Xenopus development, we have isolated a gene that is first transcribed at the mid-blastula transition. Transcription of this gene, called GS17, stops at mid-gastrula and the mRNA is rapidly degraded. Consequently, transcripts of GS17 are only present for a brief period, primarily during gastrulation. When the GS17 gene is injected into fertilized eggs, transcription from the injected DNA mimics the expression pattern of the endogenous gene, i.e., both the switch-on and switch-off of transcription are correctly regulated. The injected DNA is not significantly amplified and remains extrachromosomal. The correct expression of genes injected into Xenopus eggs will make it possible to investigate maternal factors involved in activating the embryonic genome.

Original languageEnglish (US)
Pages (from-to)3463-3471
Number of pages9
JournalThe EMBO journal
Volume4
Issue number13 A
DOIs
StatePublished - Dec 16 1985
Externally publishedYes

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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