Developmental regulation of mitochondrial biogenesis and function in the mouse mammary gland during a prolonged lactation cycle

Darryl L. Hadsell, Walter Olea, Jerry Wei, Marta L. Fiorotto, Risë K. Matsunami, David A. Engler, Robert J Collier

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The regulation of mitochondrial biogenesis and function in the lactating mammary cell is poorly understood. The goal of this study was to use proteomics to relate temporal changes in mammary cell mitochondrial function during lactation to changes in the proteins that make up this organelle. The hypothesis tested was that changes in mammary cell mitochondrial biogenesis and function during lactation would be accounted for by coordinated changes in the proteins of the electron transport chain and that some of these proteins might be linked by their expression patterns to PPARGC1α and AMP kinase. The mitochondrial proteome was studied along with markers of mitochondrial biogenesis and function in mammary tissue collected from mice over the course of a single prolonged lactation cycle. Mammary tissue concentrations of AMP and ADP were increased (P < 0.05) during early lactation and then declined with prolonged lactation. Similar changes were also observed for mitochondrial ATP synthesis activity, mitochondrial mass and DNA copy number. Analysis of the mammary cell mitochondrial proteome identified 244 unique proteins. Of these, only two proteins of the electron transport chain were found to increase during early lactation. In contrast, coordinated changes in numerous electron transport chain proteins were observed both during mid- and late lactation. There were six proteins that could be directly linked to PPARGC1α through network analysis. Abundance of PPARGC-1α and phosphorylation of AMP kinase was highest on day 2 postpartum. The results suggest that the increases in mammary mitochondria ATP synthesis activity during early lactation results from changes in only a limited number proteins. In addition, decreases in a handful of proteins linked to lipid oxidation could be temporally linked to decreases in PPARGC1α and phospho-AMP kinase suggesting potential roles for these proteins in coordinating mammary gland metabolism during early lactation.

Original languageEnglish (US)
Pages (from-to)271-285
Number of pages15
JournalPhysiological Genomics
Volume43
Issue number6
DOIs
StatePublished - Mar 2011

Fingerprint

Organelle Biogenesis
Human Mammary Glands
Lactation
Breast
Adenylate Kinase
Electron Transport
Proteins
Protein Transport
Proteome
Lipid-Linked Proteins
Adenosine Triphosphate
Adenosine Monophosphate
Mitochondrial DNA
Organelles
Proteomics
Adenosine Diphosphate
Postpartum Period
Carrier Proteins
Mitochondria
Phosphorylation

Keywords

  • Adenosine 5′-triphosphate
  • Mitochondria
  • Proteome

ASJC Scopus subject areas

  • Physiology
  • Genetics

Cite this

Developmental regulation of mitochondrial biogenesis and function in the mouse mammary gland during a prolonged lactation cycle. / Hadsell, Darryl L.; Olea, Walter; Wei, Jerry; Fiorotto, Marta L.; Matsunami, Risë K.; Engler, David A.; Collier, Robert J.

In: Physiological Genomics, Vol. 43, No. 6, 03.2011, p. 271-285.

Research output: Contribution to journalArticle

Hadsell, Darryl L. ; Olea, Walter ; Wei, Jerry ; Fiorotto, Marta L. ; Matsunami, Risë K. ; Engler, David A. ; Collier, Robert J. / Developmental regulation of mitochondrial biogenesis and function in the mouse mammary gland during a prolonged lactation cycle. In: Physiological Genomics. 2011 ; Vol. 43, No. 6. pp. 271-285.
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