Dexamethasone effects on myoblast proliferation and differentiation

Vincent Guerriero, J. R. Florini

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Effects of glucocorticoids on myoblast proliferation and differentiation were determined in cell culture. Dexamethasone (1 x 10-7 M) decreased myoblast doubling time by 7 hr and increased cell densities as much as 800% after 8 days of exposure of the cells to dexamethasone. Stimulation of myoblast proliferation by glucocorticoids was dose dependent, with half-maximal activity exhibited at concentrations of 3 x 10-9 M dexamethasone or 2.3 x 10-8 M corticosterone. In differentiating cultures, the rate of accumulation and final levels of DNA and creatine phosphokinase were highly stimulated by dexamethasone. However, normalization of creatine phosphokinase levels (a marker for myoblast differentiation) per μg DNA demonstrated a small (30%) decrease in differentiation in cultures exposed to glucocorticoids. These results indicate that glucocorticoids stimulate early myogenesis by increasing the number of myoblasts but do not stimulate the differentiation of these cells.

Original languageEnglish (US)
Pages (from-to)1198-1202
Number of pages5
JournalEndocrinology
Volume106
Issue number4
StatePublished - 1980
Externally publishedYes

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Myoblasts
Dexamethasone
Glucocorticoids
Creatine Kinase
Muscle Development
DNA
Differentiation Antigens
Corticosterone
Cell Differentiation
Cell Culture Techniques
Cell Count

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Dexamethasone effects on myoblast proliferation and differentiation. / Guerriero, Vincent; Florini, J. R.

In: Endocrinology, Vol. 106, No. 4, 1980, p. 1198-1202.

Research output: Contribution to journalArticle

Guerriero, Vincent ; Florini, J. R. / Dexamethasone effects on myoblast proliferation and differentiation. In: Endocrinology. 1980 ; Vol. 106, No. 4. pp. 1198-1202.
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