Diagnosis and characterization of insecticide-insensitive acetylcholinesterase in three populations of the sweetpotato whitefly bemisia tabaci

N. M. Anthony, Judith K Brown, R. Feyereisen, R. H. Ffrench-Constant

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

A biochemical approach was used to characterize acetylcholinesterase (ACHE) insecticide insensitivity in several sweetpotato whitefly (Bemisia tabaci; SPW) populations. Discriminating doses of insecticide were established to differentiate between sensitive and insensitive SPW strains and to genotype individual whitefly. This technique was then used to examine the frequency of insensitive AChE alleles in several SPW populations and to isolate a line homozygous for insensitive AChE from a heterogenous B-type population. Inheritance of putative altered AChE genotypes was consistent with the proposed haplo-diploid status of B. tabaci. This biochemical diagnostic was also employed to determine the role of insensitive AChE in the observed resistance profiles of several laboratory populations subjected to different selection regimes. In keeping with previous studies on insecticide resistance in SPW, resistance does not appear to be uniquely associated with the B-type but rather with SPW populations found in crop systems.

Original languageEnglish (US)
Pages (from-to)39-46
Number of pages8
JournalPesticide Science
Volume52
Issue number1
DOIs
StatePublished - 1998
Externally publishedYes

Fingerprint

Hemiptera
Bemisia tabaci
Acetylcholinesterase
acetylcholinesterase
Insecticides
insecticides
Population
Genotype
Insecticide Resistance
genotype
insecticide resistance
Aleyrodidae
Diploidy
inheritance (genetics)
diploidy
Alleles
alleles
crops
dosage

Keywords

  • Acetylcholinesterase
  • Insecticide
  • Resistance
  • Whitefly

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

Cite this

Diagnosis and characterization of insecticide-insensitive acetylcholinesterase in three populations of the sweetpotato whitefly bemisia tabaci. / Anthony, N. M.; Brown, Judith K; Feyereisen, R.; Ffrench-Constant, R. H.

In: Pesticide Science, Vol. 52, No. 1, 1998, p. 39-46.

Research output: Contribution to journalArticle

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