Diagnosis of pulmonary histoplasmosis and blastomycosis by detection of antigen in bronchoalveolar lavage fluid using an improved second-generation enzyme-linked immunoassay

Chadi A. Hage, Thomas E. Davis, Lindsey Egan, Michelle Parker, Deanna Fuller, Ann M. LeMonte, Michelle Durkin, Patricia Connelly, L. Joseph Wheat, Deborah Blue-Hnidy, Kenneth S. Knox

Research output: Contribution to journalArticle

44 Scopus citations

Abstract

Antigen detection is a useful adjunct for the diagnosis of histoplasmosis. The purpose of this study was to evaluate antigen detection in bronchoalveolar lavage (BAL) fluid using an improved second-generation Histoplasma antigen assay. Antigen was detected in 16 of 19 (84%) cases of histoplasmosis and 5 of 6 (83.3%) blastomycosis cases using the second-generation assay vs. 13 of 19 (68%) and 4 of 6 (66.7%), respectively, in the original assay. Ten-fold concentration permitted detection of antigen in an additional case of histoplasmosis and another with blastomycosis, for an overall sensitivity of 23 of 25 (92.0%). Specificity was 98.2% in both assays in controls with other pulmonary infections. These findings support the diagnostic utility of the second-generation assay in patients with pulmonary histoplasmosis and blastomycosis.

Original languageEnglish (US)
Pages (from-to)43-47
Number of pages5
JournalRespiratory Medicine
Volume101
Issue number1
DOIs
StatePublished - Jan 1 2007

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Keywords

  • Blastomycosis
  • Bronchoalveolar lavage
  • Histoplasma antigen
  • Histoplasmosis
  • Second-generation assay

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

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