Diazole and triazole inhibition of nitrification process in return activated sludge

Guangbin Li, James A. Field, Chao Zeng, Camila Leite Madeira, Chi Huynh Nguyen, Kalyani Vikas Jog, David Speed, Reyes Sierra-Alvarez

Research output: Contribution to journalArticle

Abstract

Azoles are emerging contaminants that are resistant to biodegradation during wastewater treatment. Their presence has been widely reported in wastewater effluents and receiving waters. In this work, the potential inhibition of nitrification process by six different azole compounds in wastewater treatment plants was investigated in batch bioassays. The azoles studied included three diazoles: pyrazole (Pz); 1-methylpyrazole (MePz); 3,5-dimethylpyrazole (DMePz); and three triazoles: 1,2,4-triazole (Tz); benzotriazole (BTz); and 5-methyl benzotriazole (MeBTz). The concentration of azoles causing 50% inhibition (IC50) increased (azoles became less inhibitory) in the following order (mg L−1): BTz (1.99) < MeBTz (2.18) < Pz (2.69) < Tz (3.53) < DMePz (17.3) < MePz (49.6). No clear structure-inhibitory relationships were found using Log P and pKa as structural properties. The toxicity of any given azole may be related to the role of substituent groups on disabling/enabling binding to the active sites of metallo-enzymes in nitrifying microorganisms. This is exemplified by the low toxicity of MePz, which has a cyclic N blocked by a methyl group. The observed inhibition caused to nitrifying bacteria is more severe than their cytotoxicity to other target organisms (e.g., methanogens and heterotrophic bacteria), suggesting a specific inhibition to the copper-containing enzyme, ammonium monooxygenase, in ammonia oxidizing nitrifying microorganisms.

Original languageEnglish (US)
Article number124993
JournalChemosphere
Volume241
DOIs
StatePublished - Feb 2020

Fingerprint

Nitrification
Azoles
Triazoles
Sewage
Wastewater treatment
Microorganisms
Toxicity
activated sludge
nitrification
Bacteria
Enzymes
Methanogens
toxicity
Enzyme inhibition
Bioassay
Cytotoxicity
Waste Water
Biodegradation
Structural properties
Effluents

Keywords

  • Azoles
  • Biological
  • Microbial inhibition
  • Toxicity

ASJC Scopus subject areas

  • Environmental Engineering
  • Environmental Chemistry
  • Chemistry(all)
  • Pollution
  • Health, Toxicology and Mutagenesis

Cite this

Diazole and triazole inhibition of nitrification process in return activated sludge. / Li, Guangbin; Field, James A.; Zeng, Chao; Madeira, Camila Leite; Nguyen, Chi Huynh; Jog, Kalyani Vikas; Speed, David; Sierra-Alvarez, Reyes.

In: Chemosphere, Vol. 241, 124993, 02.2020.

Research output: Contribution to journalArticle

Li, Guangbin ; Field, James A. ; Zeng, Chao ; Madeira, Camila Leite ; Nguyen, Chi Huynh ; Jog, Kalyani Vikas ; Speed, David ; Sierra-Alvarez, Reyes. / Diazole and triazole inhibition of nitrification process in return activated sludge. In: Chemosphere. 2020 ; Vol. 241.
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AU - Speed, David

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