Dibutyryl cAMP activates bumetanide-sensitive electrolyte transport in Malpighian tubules

J. L. Hegarty, B. Zhang, Thomas L Pannabecker, D. H. Petzel, M. D. Baustian, K. W. Beyenbach

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

The effects of dibutyryl adenosine 3',5'-cyclic monophosphate (DBcAMP) and bumetanide (both 10-4 M) on transepithelial Na+, K+, Cl-, and fluid secretion and on tubule electrophysiology were studied in isolated Malpighian tubules of the yellow fever mosquito Aedes aegypti. Peritubular DBcAMP significantly increased Na+, Cl-, and fluid secretion but decreased K+ secretion. In DBcAMP-stimulated tubules, bumetanide caused Na+, Cl-, and fluid secretion to return to pre-cAMP control rates and K+ secretion to decrease further. Peritubular bumetanide significantly increased Na+ secretion and decreased K+ secretion so that Cl- and fluid secretion did not change. In bumetanide-treated tubules, the secretagogue effects of DBcAMP are blocked. In isolated Malpighian tubules perfused with symmetrical Ringer solution, DBcAMP significantly hyperpolarized the transepithelial voltage (V(T)) and depolarized the basolateral membrane voltage (V(bl)) with no effect on apical membrane voltage (V(a)). Total transepithelial resistance (R(T)) and the fractional resistance of the basolateral membrane (fR(bl)) significantly decreased. Bumetanide also hyperpolarized V(T) and depolarized V(bl), however without significantly affecting R(T) and fR(bl). Together these results suggest that, in addition to stimulating electroconductive transport, DBcAMP also activates a nonconductive bumetanide-sensitive transport system in Aedes Malpighian tubules.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume261
Issue number3 30-3
StatePublished - 1991
Externally publishedYes

Fingerprint

Malpighian Tubules
Bumetanide
Bucladesine
Electrolytes
Fluids and Secretions
Membranes
Fluids
Electric potential
Aedes
Electrophysiology
Yellow Fever
Cyclic AMP
Culicidae

Keywords

  • 4-acetamido-4'-isothiocynanostilbene-2,2'- disulfonic acid
  • Aedes aegypti
  • Dibutyryl adenosine 3',5'-cyclic monophosphate
  • Electron probe analysis
  • Membrane voltage and fractional resistance
  • Ouabain
  • Secretory diuresis
  • Transepithelial sodium, potassium, and chloride secretion
  • Transepithelial voltage and resistance

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Dibutyryl cAMP activates bumetanide-sensitive electrolyte transport in Malpighian tubules. / Hegarty, J. L.; Zhang, B.; Pannabecker, Thomas L; Petzel, D. H.; Baustian, M. D.; Beyenbach, K. W.

In: American Journal of Physiology - Cell Physiology, Vol. 261, No. 3 30-3, 1991.

Research output: Contribution to journalArticle

Hegarty, J. L. ; Zhang, B. ; Pannabecker, Thomas L ; Petzel, D. H. ; Baustian, M. D. ; Beyenbach, K. W. / Dibutyryl cAMP activates bumetanide-sensitive electrolyte transport in Malpighian tubules. In: American Journal of Physiology - Cell Physiology. 1991 ; Vol. 261, No. 3 30-3.
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AU - Baustian, M. D.

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AB - The effects of dibutyryl adenosine 3',5'-cyclic monophosphate (DBcAMP) and bumetanide (both 10-4 M) on transepithelial Na+, K+, Cl-, and fluid secretion and on tubule electrophysiology were studied in isolated Malpighian tubules of the yellow fever mosquito Aedes aegypti. Peritubular DBcAMP significantly increased Na+, Cl-, and fluid secretion but decreased K+ secretion. In DBcAMP-stimulated tubules, bumetanide caused Na+, Cl-, and fluid secretion to return to pre-cAMP control rates and K+ secretion to decrease further. Peritubular bumetanide significantly increased Na+ secretion and decreased K+ secretion so that Cl- and fluid secretion did not change. In bumetanide-treated tubules, the secretagogue effects of DBcAMP are blocked. In isolated Malpighian tubules perfused with symmetrical Ringer solution, DBcAMP significantly hyperpolarized the transepithelial voltage (V(T)) and depolarized the basolateral membrane voltage (V(bl)) with no effect on apical membrane voltage (V(a)). Total transepithelial resistance (R(T)) and the fractional resistance of the basolateral membrane (fR(bl)) significantly decreased. Bumetanide also hyperpolarized V(T) and depolarized V(bl), however without significantly affecting R(T) and fR(bl). Together these results suggest that, in addition to stimulating electroconductive transport, DBcAMP also activates a nonconductive bumetanide-sensitive transport system in Aedes Malpighian tubules.

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