Dielectrophoretic characterization of erythrocytes: Positive ABO blood types

Soumya K. Srivastava, Prashant R. Daggolu, Shane C. Burgess, Adrienne R. Minerick

Research output: Contribution to journalArticle

61 Scopus citations

Abstract

Dielectrophoretic manipulation of erythrocytes/red blood cells is investigated as a tool to identify blood type for medical diagnostic applications. Positive blood types of the ABO typing system (A+, B+, AB+ and O+) were tested and cell responses quantified. The dielectrophoretic response of each blood type was observed in a platinum electrode microdevice, delivering a field of 0.025Vpp/μm at 1 MHz. Responses were recorded via video microscopy for 120 s and erythrocyte positions were tabulated at 20-30 s intervals. Both vertical and horizontal motions of erythrocytes were quantified via image object recognition, object tracking in MATLAB, binning into appropriate electric field contoured regions (wedges) and statistical analysis. Cells of O+ type showed relatively attenuated response to the dielectrophoretic field and were distinguished with greater than 95% confidence from all the other three blood types. AB+ cell responses differed from A+ and B+ blood types likely because AB+ erythrocytes express both the A and B glycoforms on their membrane. This research suggests that dielectrophoresis of untreated erythrocytes beyond simple dilution depends on blood type and could be used in portable blood typing devices.

Original languageEnglish (US)
Pages (from-to)5033-5046
Number of pages14
JournalELECTROPHORESIS
Volume29
Issue number24
DOIs
StatePublished - Dec 1 2008
Externally publishedYes

Keywords

  • ABO blood type
  • Dielectrophoresis
  • Erythrocytes
  • Medical diagnostics
  • Microdevice

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

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