Dietary Isoflavone-Dependent and Estradiol Replacement Effects on Body Weight in the Ovariectomized (OVX) Rat

Ashley L. Russell, Jamie Moran Grimes, Danette F. Cruthirds, Joanna Westerfield, Lawren Wooten, Margaret Keil, Michael J. Weiser, Michael R. Landauer, Robert J Handa, T. John Wu, Darwin O. Larco

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

17β-Estradiol is known to regulate energy metabolism and body weight. Ovariectomy results in body weight gain while estradiol administration results in a reversal of weight gain. Isoflavones, found in rodent chow, can mimic estrogenic effects making it crucial to understand the role of these compounds on metabolic regulation. The goal of this study is to examine the effect of dietary isoflavones on body weight regulation in the ovariectomized rat. This study will examine how dietary isoflavones can interact with estradiol treatment to affect body weight. Consistent with previous findings, animals fed an isoflavone-rich diet had decreased body weight (p<0.05), abdominal fat (p<0.05), and serum leptin levels (p<0.05) compared to animals fed an isoflavone-free diet. Estradiol replacement resulted in decreased body weight (p<0.05), abdominal fat (p<0.05), and serum leptin (p<0.05). Current literature suggests the involvement of cytokines in the inflammatory response of body weight gain. We screened a host of cytokines and chemokines that may be altered by dietary isoflavones or estradiol replacement. Serum cytokine analysis revealed significant (p<0.05) diet-dependent increases in inflammatory cytokines (keratinocyte-derived chemokine). The isoflavone-free diet in OVX rats resulted in the regulation of the following cytokines and chemokines: interleukin-10, interleukin-18, serum regulated on activation, normal T cell expressed and secreted, and monocyte chemoattractant protein-1 (p<0.05). Overall, these results reveal that estradiol treatment can have differential effects on energy metabolism and body weight regulation depending on the presence of isoflavones in rodent chow.

Original languageEnglish (US)
Pages (from-to)457-465
Number of pages9
JournalHormone and Metabolic Research
Volume49
Issue number6
DOIs
StatePublished - Jun 1 2017
Externally publishedYes

Fingerprint

Isoflavones
Rats
Estradiol
Body Weight
Nutrition
Cytokines
Diet
Weight Gain
Abdominal Fat
Leptin
Serum
Chemokines
Energy Metabolism
Rodentia
Animals
Fats
Interleukin-18
T-cells
Chemokine CCL2
Ovariectomy

Keywords

  • cytokines
  • energy metabolism
  • leptin

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Russell, A. L., Grimes, J. M., Cruthirds, D. F., Westerfield, J., Wooten, L., Keil, M., ... Larco, D. O. (2017). Dietary Isoflavone-Dependent and Estradiol Replacement Effects on Body Weight in the Ovariectomized (OVX) Rat. Hormone and Metabolic Research, 49(6), 457-465. https://doi.org/10.1055/s-0043-108250

Dietary Isoflavone-Dependent and Estradiol Replacement Effects on Body Weight in the Ovariectomized (OVX) Rat. / Russell, Ashley L.; Grimes, Jamie Moran; Cruthirds, Danette F.; Westerfield, Joanna; Wooten, Lawren; Keil, Margaret; Weiser, Michael J.; Landauer, Michael R.; Handa, Robert J; Wu, T. John; Larco, Darwin O.

In: Hormone and Metabolic Research, Vol. 49, No. 6, 01.06.2017, p. 457-465.

Research output: Contribution to journalArticle

Russell, AL, Grimes, JM, Cruthirds, DF, Westerfield, J, Wooten, L, Keil, M, Weiser, MJ, Landauer, MR, Handa, RJ, Wu, TJ & Larco, DO 2017, 'Dietary Isoflavone-Dependent and Estradiol Replacement Effects on Body Weight in the Ovariectomized (OVX) Rat', Hormone and Metabolic Research, vol. 49, no. 6, pp. 457-465. https://doi.org/10.1055/s-0043-108250
Russell, Ashley L. ; Grimes, Jamie Moran ; Cruthirds, Danette F. ; Westerfield, Joanna ; Wooten, Lawren ; Keil, Margaret ; Weiser, Michael J. ; Landauer, Michael R. ; Handa, Robert J ; Wu, T. John ; Larco, Darwin O. / Dietary Isoflavone-Dependent and Estradiol Replacement Effects on Body Weight in the Ovariectomized (OVX) Rat. In: Hormone and Metabolic Research. 2017 ; Vol. 49, No. 6. pp. 457-465.
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