Differences in CSF phospholipid concentration by traumatic brain injury outcome

Alice E Pasvogel, Petra Miketova, Ida M Moore

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Traumatic brain injury (TBI) is a leading cause of morbidity and mortality. A cascade of events is initiated with TBI that leads to degradation of the membrane lipid bilayer of neurons and neuroglia. The purpose of this study was to (a) describe changes in the cerebrospinal fluid (CSF) phospholipid concentration over time for those who survived and those who died following TBI; and (b) determine whether there were differences in the CSF phospholipid concentration between those who survived and those who died following TBI. Thirty-nine CSF samples were obtained from 10 participants who sustained a TBI. Following extraction, phospholipids were separated and quantified by normal-phase high performance liquid chromatography with ultraviolet detector. For those who died, the highest median concentration was on Day 1 after TBI for lysophosphatidylcholine and on Day 4 after TBI for phosphatidylethanolamine, phosphatidylserine, phosphatidylcholine, and sphingomyelin. For those who survived, the highest median concentration was on Day 1 after TBI for phosphatidylcholine, on Day 3 after TBI for phosphatidylethanolamine and phosphatidylserine, on Day 4 after TBI for sphingomyelin, and on Day 5 after TBI for lysophosphatidylcholine. There were significant differences in the concentrations of phosphatidylethanolamine and phosphatidylserine on Days 1-2 and of phosphatidylethanolamine, phosphatidylcholine, and sphingomyelin on Days 3-4 after TBI between those who survived and died, with the highest concentrations in those who died. These findings provide preliminary evidence of greater disruption of central nervous system membrane phospholipids in participants who died after TBI.

Original languageEnglish (US)
Pages (from-to)325-331
Number of pages7
JournalBiological Research for Nursing
Volume11
Issue number4
DOIs
StatePublished - Apr 2010

Fingerprint

Cerebrospinal Fluid
Phospholipids
Sphingomyelins
Phosphatidylserines
Phosphatidylcholines
Lysophosphatidylcholines
Traumatic Brain Injury
Lipid Bilayers
Membrane Lipids
Neuroglia
Central Nervous System
High Pressure Liquid Chromatography
Morbidity
Neurons
Membranes
Mortality
phosphatidylethanolamine

Keywords

  • Membrane damage
  • Outcome
  • Phospholipids
  • Traumatic brain injury

ASJC Scopus subject areas

  • Research and Theory

Cite this

Differences in CSF phospholipid concentration by traumatic brain injury outcome. / Pasvogel, Alice E; Miketova, Petra; Moore, Ida M.

In: Biological Research for Nursing, Vol. 11, No. 4, 04.2010, p. 325-331.

Research output: Contribution to journalArticle

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