Different endocytic functions of AGEF-1 in C. elegans coelomocytes

Lichun Tang, Hanna F Fares, Xingfu Zhao, Wei Du, Bi Feng Liu

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background: ADP-ribosylation factors (ARFs) are a family of small GTP-binding proteins that play roles in membrane dynamics and vesicle trafficking. AGEF-1, which is thought to act as a guanine nucleotide exchange factor of class I ARFs, is required for caveolin-1 body formation and receptor-mediated endocytosis in oocytes of Caenorhabditis elegans. This study explores additional roles of AGEF-1 in endocytic transport. Methods: agef-1 expression was knocked down by using RNAi in C. elegans. Markers that allow analysis of endocytic transport in scavenger cells were investigated for studying the effect of AGEF-1 on different steps of membrane transport. Results: Knockdown of AGEF-1 levels results in two apparent trafficking defects in coelomocytes of C. elegans. First, there is a delay in the uptake of solutes from the extracellular medium. Second, there is a dramatic enlargement of the sizes of lysosomes, even though lysosomal acidification is normal and degradation still occurs. Conclusion: Our results suggest that AGEF-1 regulates endosome/lysosome fusion or fission events, in addition to earlier steps in endocytic transport. General significance: AGEF-1 is the first identified GTPase regulator that functions at the lysosome fusion or fission stage of the endocytic pathway. Our study provides insight into lysosome dynamics in C. elegans.

Original languageEnglish (US)
Pages (from-to)829-840
Number of pages12
JournalBBA - General Subjects
Volume1820
Issue number7
DOIs
StatePublished - Jul 2012

Fingerprint

ADP-Ribosylation Factors
Caenorhabditis elegans
Lysosomes
Fusion reactions
Membranes
Guanine Nucleotide Exchange Factors
Caveolin 1
Acidification
GTP Phosphohydrolases
GTP-Binding Proteins
Degradation
Defects
Endosomes
RNA Interference
Endocytosis
Oocytes

Keywords

  • AGEF-1
  • ARF-GEF
  • ARL-8
  • C. elegans
  • Endosomes
  • Lysosomes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Different endocytic functions of AGEF-1 in C. elegans coelomocytes. / Tang, Lichun; Fares, Hanna F; Zhao, Xingfu; Du, Wei; Liu, Bi Feng.

In: BBA - General Subjects, Vol. 1820, No. 7, 07.2012, p. 829-840.

Research output: Contribution to journalArticle

Tang, Lichun ; Fares, Hanna F ; Zhao, Xingfu ; Du, Wei ; Liu, Bi Feng. / Different endocytic functions of AGEF-1 in C. elegans coelomocytes. In: BBA - General Subjects. 2012 ; Vol. 1820, No. 7. pp. 829-840.
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