Abstract
Background: ADP-ribosylation factors (ARFs) are a family of small GTP-binding proteins that play roles in membrane dynamics and vesicle trafficking. AGEF-1, which is thought to act as a guanine nucleotide exchange factor of class I ARFs, is required for caveolin-1 body formation and receptor-mediated endocytosis in oocytes of Caenorhabditis elegans. This study explores additional roles of AGEF-1 in endocytic transport. Methods: agef-1 expression was knocked down by using RNAi in C. elegans. Markers that allow analysis of endocytic transport in scavenger cells were investigated for studying the effect of AGEF-1 on different steps of membrane transport. Results: Knockdown of AGEF-1 levels results in two apparent trafficking defects in coelomocytes of C. elegans. First, there is a delay in the uptake of solutes from the extracellular medium. Second, there is a dramatic enlargement of the sizes of lysosomes, even though lysosomal acidification is normal and degradation still occurs. Conclusion: Our results suggest that AGEF-1 regulates endosome/lysosome fusion or fission events, in addition to earlier steps in endocytic transport. General significance: AGEF-1 is the first identified GTPase regulator that functions at the lysosome fusion or fission stage of the endocytic pathway. Our study provides insight into lysosome dynamics in C. elegans.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 829-840 |
| Number of pages | 12 |
| Journal | BBA - General Subjects |
| Volume | 1820 |
| Issue number | 7 |
| DOIs | |
| State | Published - Jul 2012 |
Fingerprint
Keywords
- AGEF-1
- ARF-GEF
- ARL-8
- C. elegans
- Endosomes
- Lysosomes
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Different endocytic functions of AGEF-1 in C. elegans coelomocytes. / Tang, Lichun; Fares, Hanna F; Zhao, Xingfu; Du, Wei; Liu, Bi Feng.
In: BBA - General Subjects, Vol. 1820, No. 7, 07.2012, p. 829-840.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Different endocytic functions of AGEF-1 in C. elegans coelomocytes
AU - Tang, Lichun
AU - Fares, Hanna F
AU - Zhao, Xingfu
AU - Du, Wei
AU - Liu, Bi Feng
PY - 2012/7
Y1 - 2012/7
N2 - Background: ADP-ribosylation factors (ARFs) are a family of small GTP-binding proteins that play roles in membrane dynamics and vesicle trafficking. AGEF-1, which is thought to act as a guanine nucleotide exchange factor of class I ARFs, is required for caveolin-1 body formation and receptor-mediated endocytosis in oocytes of Caenorhabditis elegans. This study explores additional roles of AGEF-1 in endocytic transport. Methods: agef-1 expression was knocked down by using RNAi in C. elegans. Markers that allow analysis of endocytic transport in scavenger cells were investigated for studying the effect of AGEF-1 on different steps of membrane transport. Results: Knockdown of AGEF-1 levels results in two apparent trafficking defects in coelomocytes of C. elegans. First, there is a delay in the uptake of solutes from the extracellular medium. Second, there is a dramatic enlargement of the sizes of lysosomes, even though lysosomal acidification is normal and degradation still occurs. Conclusion: Our results suggest that AGEF-1 regulates endosome/lysosome fusion or fission events, in addition to earlier steps in endocytic transport. General significance: AGEF-1 is the first identified GTPase regulator that functions at the lysosome fusion or fission stage of the endocytic pathway. Our study provides insight into lysosome dynamics in C. elegans.
AB - Background: ADP-ribosylation factors (ARFs) are a family of small GTP-binding proteins that play roles in membrane dynamics and vesicle trafficking. AGEF-1, which is thought to act as a guanine nucleotide exchange factor of class I ARFs, is required for caveolin-1 body formation and receptor-mediated endocytosis in oocytes of Caenorhabditis elegans. This study explores additional roles of AGEF-1 in endocytic transport. Methods: agef-1 expression was knocked down by using RNAi in C. elegans. Markers that allow analysis of endocytic transport in scavenger cells were investigated for studying the effect of AGEF-1 on different steps of membrane transport. Results: Knockdown of AGEF-1 levels results in two apparent trafficking defects in coelomocytes of C. elegans. First, there is a delay in the uptake of solutes from the extracellular medium. Second, there is a dramatic enlargement of the sizes of lysosomes, even though lysosomal acidification is normal and degradation still occurs. Conclusion: Our results suggest that AGEF-1 regulates endosome/lysosome fusion or fission events, in addition to earlier steps in endocytic transport. General significance: AGEF-1 is the first identified GTPase regulator that functions at the lysosome fusion or fission stage of the endocytic pathway. Our study provides insight into lysosome dynamics in C. elegans.
KW - AGEF-1
KW - ARF-GEF
KW - ARL-8
KW - C. elegans
KW - Endosomes
KW - Lysosomes
UR - http://www.scopus.com/inward/record.url?scp=84860855011&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84860855011&partnerID=8YFLogxK
U2 - 10.1016/j.bbagen.2012.03.004
DO - 10.1016/j.bbagen.2012.03.004
M3 - Article
C2 - 22446376
AN - SCOPUS:84860855011
VL - 1820
SP - 829
EP - 840
JO - Biochimica et Biophysica Acta - General Subjects
JF - Biochimica et Biophysica Acta - General Subjects
SN - 0304-4165
IS - 7
ER -