Different signaling pathways expressed by chicken naïve CD4 + T cells, CD4 + lymphocytes activated with staphylococcal enterotoxin B, and those malignantly transformed by marek's disease virus

Joram J. Buza, Shane C Burgess

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Proteomics methods, based on liquid chromatography and tandem mass spectrometry, produce large "shotgun" proteomes that are most appropriately compared not at the level of differentially expressed proteins only but at the more comprehensive level of biological networks and pathways. This is now possible with the emergence of functional annotation databases and tools, databases of canonical pathways and molecular interactions and computational text mining tools. Here, we used shotgun proteomics, and the differential proteomics modeling functionalities available in the Pathwaystudio network modeling program to define the cell physiology of Hodgkin's disease antigen-overexpressing (CD30 hi) CD4 + T cell lymphomas using the unique Marek's disease (MD) natural animal model. CD30 hi lymphoma cells have characteristics of activated T cells but are also fundamentally different from their nontransformed healthy counterparts. We compared the cell physiology of naïve, superantigen-activated and MD-transformed CD4 + T cell proteomes. While the superantigen- activated cells had signaling pathways associated with cell activation, inflammation, proliferation and cell death, the MD-transformed cells had growth factor, cytokine, adhesion, and transcription factor signaling responses associated with oncogenicity, cell proliferation, angiogenesis, motility, and metastasis.

Original languageEnglish (US)
Pages (from-to)2380-2387
Number of pages8
JournalJournal of Proteome Research
Volume7
Issue number6
DOIs
StatePublished - Jun 2008
Externally publishedYes

Fingerprint

Marek Disease
T-cells
Lymphocytes
Viruses
Chickens
Proteomics
T-Lymphocytes
Cell Physiological Phenomena
Superantigens
Physiology
Firearms
Proteome
CD30 Antigens
Databases
Cell signaling
Data Mining
Molecular interactions
T-Cell Lymphoma
Liquid chromatography
Cell proliferation

Keywords

  • CD4 T cells
  • Differential proteomics
  • Enterotoxin B
  • Lymphoma
  • Marek's disease
  • MudPIT
  • Pathwaystudio
  • Shotgun proteomics
  • Staphylococcus

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)
  • Genetics
  • Biotechnology

Cite this

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title = "Different signaling pathways expressed by chicken na{\"i}ve CD4 + T cells, CD4 + lymphocytes activated with staphylococcal enterotoxin B, and those malignantly transformed by marek's disease virus",
abstract = "Proteomics methods, based on liquid chromatography and tandem mass spectrometry, produce large {"}shotgun{"} proteomes that are most appropriately compared not at the level of differentially expressed proteins only but at the more comprehensive level of biological networks and pathways. This is now possible with the emergence of functional annotation databases and tools, databases of canonical pathways and molecular interactions and computational text mining tools. Here, we used shotgun proteomics, and the differential proteomics modeling functionalities available in the Pathwaystudio network modeling program to define the cell physiology of Hodgkin's disease antigen-overexpressing (CD30 hi) CD4 + T cell lymphomas using the unique Marek's disease (MD) natural animal model. CD30 hi lymphoma cells have characteristics of activated T cells but are also fundamentally different from their nontransformed healthy counterparts. We compared the cell physiology of na{\"i}ve, superantigen-activated and MD-transformed CD4 + T cell proteomes. While the superantigen- activated cells had signaling pathways associated with cell activation, inflammation, proliferation and cell death, the MD-transformed cells had growth factor, cytokine, adhesion, and transcription factor signaling responses associated with oncogenicity, cell proliferation, angiogenesis, motility, and metastasis.",
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