Differential Cotton leaf crumple virus-VIGS-mediated gene silencing and viral genome localization in different Gossypium hirsutum genetic backgrounds

Ali M. Idris, J. R. Tuttle, D. Robertson, C. H. Haigler, J. K. Brown

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

A Cotton leaf crumple virus (CLCrV)-based gene silencing vector containing a fragment of the Gossypium hirsutum Magnesium chelatase subunit I was used to establish endogenous gene silencing in cotton of varied genetic backgrounds. Biolistic inoculation resulted in systemic and persistent photo-bleaching of the leaves and bolls of the seven cultivars tested, however, the intensity of silencing was variable. CLCrV-VIGS-mediated expression of green fluorescent protein was used to monitor the in planta distribution of the vector, indicating successful phloem invasion in all cultivars tested. Acala SJ-1, one of the cotton cultivars, was identified as a particularly optimal candidate for CLCrV-VIGS-based cotton reverse-genetics.

Original languageEnglish (US)
Pages (from-to)13-22
Number of pages10
JournalPhysiological and Molecular Plant Pathology
Volume75
Issue number1-2
DOIs
StatePublished - Dec 1 2010

Keywords

  • Begomovirus
  • Cotton breeding
  • Cotton genetics
  • Geminivirus
  • Genetic improvement
  • Magnesium chelatase marker
  • Viral episome

ASJC Scopus subject areas

  • Genetics
  • Plant Science

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