Differential detergent fractionation for non-electrophoretic bovine peripheral blood monocyte proteomics reveals proteins involved in professional antigen presentation

S. R. Lee, G. T. Pharr, A. M. Cooksey, Fiona M McCarthy, B. L. Boyd, L. M. Pinchuk

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Professional antigen presenting cells (APC), dendritic cells (DC) and their myeloid progenitors, monocytes/macrophages are critical controllers of innate and adaptive immunity. Here we show that differential detergent fractionation (DDF) analysis of bovine monocytes reveals proteins related to antigen pattern recognition, uptake and presentation to immunocompetent lymphocytes. We identify 53 bovine proteins involved in immune function of professional APC. In particular, 13 adhesion molecules, three toll-like receptors (TLR1, 6 and 8), three antigen uptake-related proteins (including mannose receptor [MR] precursor), and eight actin-like proteins involved in active endocytosis were identified. In addition, MHC class I and II-related proteins, cytokines, active substances and growth factors have been identified. We conclude that the DDF approach can provide interpretable and meaningful functional information concerning protein expression profiles associated with monocyte activation, transformation into macrophages and/or immature DC, and maturation of monocyte-derived DC in the presence of multiple bovine pathogens.

Original languageEnglish (US)
Pages (from-to)1070-1083
Number of pages14
JournalDevelopmental and Comparative Immunology
Volume30
Issue number11
DOIs
StatePublished - 2006
Externally publishedYes

Fingerprint

Antigen Presentation
Detergents
Proteomics
Monocytes
Dendritic Cells
Proteins
Antigen-Presenting Cells
Toll-Like Receptor 6
Toll-Like Receptor 8
Macrophages
Antigens
Adaptive Immunity
Endocytosis
Innate Immunity
Actins
Intercellular Signaling Peptides and Proteins
Lymphocytes
Cytokines

Keywords

  • Actin
  • Cytokines
  • DC
  • DDF
  • Growth factors
  • Lectin
  • MHC
  • Monocytes
  • Progenitors
  • TLR

ASJC Scopus subject areas

  • Developmental Biology
  • Immunology

Cite this

Differential detergent fractionation for non-electrophoretic bovine peripheral blood monocyte proteomics reveals proteins involved in professional antigen presentation. / Lee, S. R.; Pharr, G. T.; Cooksey, A. M.; McCarthy, Fiona M; Boyd, B. L.; Pinchuk, L. M.

In: Developmental and Comparative Immunology, Vol. 30, No. 11, 2006, p. 1070-1083.

Research output: Contribution to journalArticle

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