Differential distribution of cytokeratins after microinjection of anti-cytokeratin monoclonal antibodies.

K. Boller, R. Kemler, H. Baribault, Thomas C Doetschman

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

In order to investigate the relationship of different cytokeratins within one cell, monoclonal antibodies directed against three trophectoderm cytokeratins TROMA 1, 2 and 3 were microinjected into mouse teratocarcinoma-derived trophoblastoma cells and indirect immunofluorescence tests were used to follow the subsequent localization of their respective antigens Endo A, B and C. Microinjection of TROMA 1 or 2 resulted in the perinuclear collapse of Endo A, B and C-containing filaments. Microinjection of TROMA 3 resulted in the perinuclear collapse of filaments containing Endo A and B, whereas Endo C condensed into cytoplasmic aggregates which appear as speckles in the fluorescence microscope. The speckles were electron microscopically located using indirect gold-labeling techniques and had a dense, granulous structure. They were often found to be associated with microtubules, although colchicine treatment before microinjection did not interfere with speckle formation. These experiments demonstrate that cytokeratins can become differentially distributed within the cytoplasm after microinjection of an anti-cytokeratin monoclonal antibody. Since Endo A is a type II cytokeratin and Endo B and C are type I cytokeratins, these results suggest that different members of one cytokeratin subfamily may be associated with cytokeratin filaments which have different functions within the same cell.

Original languageEnglish (US)
Pages (from-to)459-468
Number of pages10
JournalEuropean Journal of Cell Biology
Volume43
Issue number3
StatePublished - Jun 1987
Externally publishedYes

Fingerprint

Microinjections
Keratins
Monoclonal Antibodies
Teratocarcinoma
Keratin-18
Colchicine
Indirect Fluorescent Antibody Technique
Microtubules
Gold
Cytoplasm
Fluorescence
Electrons
Antigens

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy

Cite this

Differential distribution of cytokeratins after microinjection of anti-cytokeratin monoclonal antibodies. / Boller, K.; Kemler, R.; Baribault, H.; Doetschman, Thomas C.

In: European Journal of Cell Biology, Vol. 43, No. 3, 06.1987, p. 459-468.

Research output: Contribution to journalArticle

@article{bda89b4e2d2c4b3e8c10dd400d77cad3,
title = "Differential distribution of cytokeratins after microinjection of anti-cytokeratin monoclonal antibodies.",
abstract = "In order to investigate the relationship of different cytokeratins within one cell, monoclonal antibodies directed against three trophectoderm cytokeratins TROMA 1, 2 and 3 were microinjected into mouse teratocarcinoma-derived trophoblastoma cells and indirect immunofluorescence tests were used to follow the subsequent localization of their respective antigens Endo A, B and C. Microinjection of TROMA 1 or 2 resulted in the perinuclear collapse of Endo A, B and C-containing filaments. Microinjection of TROMA 3 resulted in the perinuclear collapse of filaments containing Endo A and B, whereas Endo C condensed into cytoplasmic aggregates which appear as speckles in the fluorescence microscope. The speckles were electron microscopically located using indirect gold-labeling techniques and had a dense, granulous structure. They were often found to be associated with microtubules, although colchicine treatment before microinjection did not interfere with speckle formation. These experiments demonstrate that cytokeratins can become differentially distributed within the cytoplasm after microinjection of an anti-cytokeratin monoclonal antibody. Since Endo A is a type II cytokeratin and Endo B and C are type I cytokeratins, these results suggest that different members of one cytokeratin subfamily may be associated with cytokeratin filaments which have different functions within the same cell.",
author = "K. Boller and R. Kemler and H. Baribault and Doetschman, {Thomas C}",
year = "1987",
month = "6",
language = "English (US)",
volume = "43",
pages = "459--468",
journal = "European Journal of Cell Biology",
issn = "0171-9335",
publisher = "Urban und Fischer Verlag GmbH und Co. KG",
number = "3",

}

TY - JOUR

T1 - Differential distribution of cytokeratins after microinjection of anti-cytokeratin monoclonal antibodies.

AU - Boller, K.

AU - Kemler, R.

AU - Baribault, H.

AU - Doetschman, Thomas C

PY - 1987/6

Y1 - 1987/6

N2 - In order to investigate the relationship of different cytokeratins within one cell, monoclonal antibodies directed against three trophectoderm cytokeratins TROMA 1, 2 and 3 were microinjected into mouse teratocarcinoma-derived trophoblastoma cells and indirect immunofluorescence tests were used to follow the subsequent localization of their respective antigens Endo A, B and C. Microinjection of TROMA 1 or 2 resulted in the perinuclear collapse of Endo A, B and C-containing filaments. Microinjection of TROMA 3 resulted in the perinuclear collapse of filaments containing Endo A and B, whereas Endo C condensed into cytoplasmic aggregates which appear as speckles in the fluorescence microscope. The speckles were electron microscopically located using indirect gold-labeling techniques and had a dense, granulous structure. They were often found to be associated with microtubules, although colchicine treatment before microinjection did not interfere with speckle formation. These experiments demonstrate that cytokeratins can become differentially distributed within the cytoplasm after microinjection of an anti-cytokeratin monoclonal antibody. Since Endo A is a type II cytokeratin and Endo B and C are type I cytokeratins, these results suggest that different members of one cytokeratin subfamily may be associated with cytokeratin filaments which have different functions within the same cell.

AB - In order to investigate the relationship of different cytokeratins within one cell, monoclonal antibodies directed against three trophectoderm cytokeratins TROMA 1, 2 and 3 were microinjected into mouse teratocarcinoma-derived trophoblastoma cells and indirect immunofluorescence tests were used to follow the subsequent localization of their respective antigens Endo A, B and C. Microinjection of TROMA 1 or 2 resulted in the perinuclear collapse of Endo A, B and C-containing filaments. Microinjection of TROMA 3 resulted in the perinuclear collapse of filaments containing Endo A and B, whereas Endo C condensed into cytoplasmic aggregates which appear as speckles in the fluorescence microscope. The speckles were electron microscopically located using indirect gold-labeling techniques and had a dense, granulous structure. They were often found to be associated with microtubules, although colchicine treatment before microinjection did not interfere with speckle formation. These experiments demonstrate that cytokeratins can become differentially distributed within the cytoplasm after microinjection of an anti-cytokeratin monoclonal antibody. Since Endo A is a type II cytokeratin and Endo B and C are type I cytokeratins, these results suggest that different members of one cytokeratin subfamily may be associated with cytokeratin filaments which have different functions within the same cell.

UR - http://www.scopus.com/inward/record.url?scp=0023354488&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023354488&partnerID=8YFLogxK

M3 - Article

C2 - 2441990

AN - SCOPUS:0023354488

VL - 43

SP - 459

EP - 468

JO - European Journal of Cell Biology

JF - European Journal of Cell Biology

SN - 0171-9335

IS - 3

ER -