Differential effects of acute ethanol treatment on cardiac contractile function in young adult and senescent mice

Jiaqi Shi, Douglas F. Larson, Bo Yang, Kyler Hunter, Mark Gorman, Sergio Montes, Julie Beischel, Ronald R. Watson

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

It is understood that marked biochemical, molecular, and performance alterations occur in cardiovascular tissues related to aging. It is logical, therefore, that differences in the cardiovascular response to ethanol consumption, when comparing younger with older individuals, may exist. We compared the left ventricular function of 6- and 15-month-old (senescent) mice and 16-month-old (senescent) inducible nitric oxide synthase knockout mice (n = 7 each) before and subsequent to acute treatment with 60% ethanol (2 g/kg, i.p.). A Millar 1.4 Fr conductance/micromanometer catheter was placed into the left ventricle of the mice for acquisition of pressure-volume loops. Heart contractile functions were significantly decreased in the senescent group, compared with findings in the younger mice. Subsequent to ethanol treatment, the younger mice showed a significant reduction in cardiac function, with a 28% decrease in cardiac index, a 29% decrease in end-systolic elastance, and a 16% decrease in preload recruitable stroke work (P <.01). Conversely, the senescent mice showed significantly increased contractile function, with a 40% increase in end-systolic elastance (P <.01) and a 19% increase in preload recruitable stroke work (P <.05). The myocardial cyclic guanosine monophosphate levels were significantly higher in the older group (P <.002), and subsequent to ethanol treatment, they were decreased by 68.5% (P <.001). Northern blot analysis demonstrated inducible nitric oxide synthase message only in senescent myocardial tissues. Moreover, the cardiac function of senescent inducible nitric oxide synthase knockout mice was comparable with that of young mice, and after ethanol treatment, cardiac function decreased significantly, just as that for young mice did, with a 26% decrease in cardiac index (P <.05) and a 23% decrease in preload recruitable stroke work (P <.01). It was concluded that the differential cardiovascular function and response to acute ethanol administration was related to the inducible nitric oxide synthase activity present only in senescent mice.

Original languageEnglish (US)
Pages (from-to)197-204
Number of pages8
JournalAlcohol
Volume24
Issue number3
DOIs
StatePublished - Sep 17 2001

Keywords

  • Aging
  • Alcohol
  • Cardiac contractility
  • Nitric oxide
  • cGMP

ASJC Scopus subject areas

  • Health(social science)
  • Biochemistry
  • Toxicology
  • Neurology
  • Behavioral Neuroscience

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