Differential effects of ethanol on GABAA and glycine receptor-mediated synaptic currents in brain stem motoneurons

Joy Y. Sebe, Erika D Eggers, Albert J. Berger

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Ethanol potentiates glycinergic synaptic transmission to hypoglossal motoneurons (HMs). This effect on glycinergic transmission changes with postnatal development in that juvenile HMs (P9-13) are more sensitive to ethanol than neonate HMs (P1-3). We have now extended our previous study to investigate ethanol modulation of synaptic GABAA receptors (GABA ARs), because both GABA and glycine mediate inhibitory synaptic transmission to brain stem motoneurons. We tested the effects of ethanol on GABAergic and glycinergic miniature inhibitory postsynaptic currents (mIPSCs) recorded from neonate and juvenile rat HMs in an in vitro slice preparation. Bath application of 30 mM ethanol had no significant effect on the GABAergic mIPSC amplitude or frequency recorded at either age. At 100 mM, ethanol significantly decreased the GABAergic mIPSC amplitude recorded from neonate (6 ± 3%, P < 0.05) and juvenile (16 ± 3%, P < 0.01) HMs. The same concentration of ethanol increased the GABAergic mIPSC frequency recorded from neonate (64 ± 17%, P < 0.05) and juvenile (40 ± 15%, n.s.) HMs. In contrast, 100 mM ethanol robustly potentiated glycinergic mIPSC amplitude in neonate (31 ± 3%, P < 0.0001) and juvenile (41 ± 7%, P < 0.001) HMs. These results suggest that glycine receptors are more sensitive to modulation by ethanol than GABAA receptors and that 100 mM ethanol has the opposite effect on GABAAR-mediated currents in juvenile HMs, that is, inhibition rather than enhancement. Further, comparing ethanol's effects on GABAergic mIPSC amplitude and frequency, ethanol modulates GABAergic synaptic transmission to HMs differentially. Presynaptically, ethanol enhances mIPSC frequency while postsynaptically it decreases mIPSC amplitude.

Original languageEnglish (US)
Pages (from-to)870-875
Number of pages6
JournalJournal of Neurophysiology
Volume90
Issue number2
DOIs
StatePublished - Aug 1 2003
Externally publishedYes

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Glycine Receptors
Motor Neurons
GABA-A Receptors
Brain Stem
Ethanol
Inhibitory Postsynaptic Potentials
Synaptic Transmission
Neurotransmitter Receptor
Baths
Glycine
gamma-Aminobutyric Acid

ASJC Scopus subject areas

  • Physiology
  • Neuroscience(all)

Cite this

Differential effects of ethanol on GABAA and glycine receptor-mediated synaptic currents in brain stem motoneurons. / Sebe, Joy Y.; Eggers, Erika D; Berger, Albert J.

In: Journal of Neurophysiology, Vol. 90, No. 2, 01.08.2003, p. 870-875.

Research output: Contribution to journalArticle

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abstract = "Ethanol potentiates glycinergic synaptic transmission to hypoglossal motoneurons (HMs). This effect on glycinergic transmission changes with postnatal development in that juvenile HMs (P9-13) are more sensitive to ethanol than neonate HMs (P1-3). We have now extended our previous study to investigate ethanol modulation of synaptic GABAA receptors (GABA ARs), because both GABA and glycine mediate inhibitory synaptic transmission to brain stem motoneurons. We tested the effects of ethanol on GABAergic and glycinergic miniature inhibitory postsynaptic currents (mIPSCs) recorded from neonate and juvenile rat HMs in an in vitro slice preparation. Bath application of 30 mM ethanol had no significant effect on the GABAergic mIPSC amplitude or frequency recorded at either age. At 100 mM, ethanol significantly decreased the GABAergic mIPSC amplitude recorded from neonate (6 ± 3{\%}, P < 0.05) and juvenile (16 ± 3{\%}, P < 0.01) HMs. The same concentration of ethanol increased the GABAergic mIPSC frequency recorded from neonate (64 ± 17{\%}, P < 0.05) and juvenile (40 ± 15{\%}, n.s.) HMs. In contrast, 100 mM ethanol robustly potentiated glycinergic mIPSC amplitude in neonate (31 ± 3{\%}, P < 0.0001) and juvenile (41 ± 7{\%}, P < 0.001) HMs. These results suggest that glycine receptors are more sensitive to modulation by ethanol than GABAA receptors and that 100 mM ethanol has the opposite effect on GABAAR-mediated currents in juvenile HMs, that is, inhibition rather than enhancement. Further, comparing ethanol's effects on GABAergic mIPSC amplitude and frequency, ethanol modulates GABAergic synaptic transmission to HMs differentially. Presynaptically, ethanol enhances mIPSC frequency while postsynaptically it decreases mIPSC amplitude.",
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