Differential effects of spermine and its analogues on the structures of polynucleotides complexed with ethidium bromide

J. G. Delcros, M. C J M Sturkenboom, H. S. Basu, R. H. Shafer, J. Szollosi, Burt G.F. Feuerstein, L. J. Marton

Research output: Contribution to journalArticle

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Abstract

The interactions of spermine and polyamine analogues with synthetic polynucleotides of various base sequences complexed with ethidium bromide (EB) were investigated using measurements of fluorescence intensity and steady-state fluorescence polarization. Spermine and polyamine analogues displaced some but not all of the EB bound to poly(dA-dT) poly(dA-dT) or poly(dG-dC) poly(dG-dC), suggesting that polyamines may stabilize these polynucleotides in a conformation with reduced affinity for EB. Modifications of the aliphatic backbone of spermine have pronounced effects on its ability to displace EB from poly(dA-dT) poly(dA-dT) but not from poly (dG-dC) poly(dG-dC). Spermine and some but not all of the polyamine analogues caused fluorescence depolarization when they interacted with the complex of EB and poly(dA-dT) poly(dA-dT). Neither spermine nor any of the analogues, however, induced fluorescence depolarization in the complex of EB with poly(dG-dC) poly(dG-dC) or poly(dA) poly(dT). This suggests that spermine and some spermine analogues induce structural changes specific to alternating A-T sequences.

Original languageEnglish (US)
Pages (from-to)269-274
Number of pages6
JournalBiochemical Journal
Volume291
Issue number1
StatePublished - 1993
Externally publishedYes

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Polynucleotides
Ethidium
Spermine
Poly dA-dT
Polyamines
Fluorescence
Depolarization
Fluorescence Polarization
Conformations
Polarization
polydeoxyadenylic acid-polythymidylic acid

ASJC Scopus subject areas

  • Biochemistry

Cite this

Delcros, J. G., Sturkenboom, M. C. J. M., Basu, H. S., Shafer, R. H., Szollosi, J., Feuerstein, B. G. F., & Marton, L. J. (1993). Differential effects of spermine and its analogues on the structures of polynucleotides complexed with ethidium bromide. Biochemical Journal, 291(1), 269-274.

Differential effects of spermine and its analogues on the structures of polynucleotides complexed with ethidium bromide. / Delcros, J. G.; Sturkenboom, M. C J M; Basu, H. S.; Shafer, R. H.; Szollosi, J.; Feuerstein, Burt G.F.; Marton, L. J.

In: Biochemical Journal, Vol. 291, No. 1, 1993, p. 269-274.

Research output: Contribution to journalArticle

Delcros, JG, Sturkenboom, MCJM, Basu, HS, Shafer, RH, Szollosi, J, Feuerstein, BGF & Marton, LJ 1993, 'Differential effects of spermine and its analogues on the structures of polynucleotides complexed with ethidium bromide', Biochemical Journal, vol. 291, no. 1, pp. 269-274.
Delcros, J. G. ; Sturkenboom, M. C J M ; Basu, H. S. ; Shafer, R. H. ; Szollosi, J. ; Feuerstein, Burt G.F. ; Marton, L. J. / Differential effects of spermine and its analogues on the structures of polynucleotides complexed with ethidium bromide. In: Biochemical Journal. 1993 ; Vol. 291, No. 1. pp. 269-274.
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