Differential expression and interaction of host factors augment HIV-1 gene expression in neonatal mononuclear cells

Vasudha Sundaravaradan, Roshni Mehta, David T. Harris, Jerome A. Zack, Nafees - Ahmad

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We have previously shown a higher level of HIV-1 replication and gene expression in neonatal (cord) blood mononuclear cells (CBMC) compared with adult blood cells (PBMC), which could be due to differential expression of host factors. We performed the gene expression profile of CBMC and PBMC and found that 8013 genes were expressed at higher levels in CBMC than PBMC and 8028 genes in PBMC than CBMC, including 1181 and 1414 genes upregulated after HIV-1 infection in CBMC and PBMC, respectively. Several transcription factors (NF-κB, E2F, HAT-1, TFIIE, Cdk9, Cyclin T1), signal transducers (STAT3, STAT5A) and cytokines (IL-1β, IL-6, IL-10) were upregulated in CBMC than PBMC, which are known to influence HIV-1 replication. In addition, a repressor of HIV-1 transcription, YY1, was down regulated in CBMC than PBMC and several matrix metalloproteinase (MMP-7, -12, -14) were significantly upregulated in HIV-1 infected CBMC than PBMC. Furthermore, we show that CBMC nuclear extracts interacted with a higher extent to HIV-1 LTR cis-acting sequences, including NF-κB, NFAT, AP1 and NF-IL6 compared with PBMC nuclear extracts and retroviral based short hairpin RNA (shRNA) for STAT3 and IL-6 down regulated their own and HIV-1 gene expression, signifying that these factors influenced differential HIV-1 gene expression in CBMC than PBMC.

Original languageEnglish (US)
Pages (from-to)32-43
Number of pages12
JournalVirology
Volume400
Issue number1
DOIs
StatePublished - Apr 25 2010

Fingerprint

Fetal Blood
HIV-1
Blood Cells
Gene Expression
Interleukin-6
Cyclin T
HIV Long Terminal Repeat
Matrix Metalloproteinase 7
CCAAT-Enhancer-Binding Protein-beta
Genes
Cell Extracts
Transducers
Matrix Metalloproteinases
Interleukin-1
Transcriptome
Interleukin-10
Small Interfering RNA
HIV Infections
Transcription Factors
Cytokines

Keywords

  • Cord and adult blood mononuclear cells
  • Cytokines
  • HIV-1 gene expression
  • Host factors
  • shRNA
  • Transcriptional factors

ASJC Scopus subject areas

  • Virology

Cite this

Differential expression and interaction of host factors augment HIV-1 gene expression in neonatal mononuclear cells. / Sundaravaradan, Vasudha; Mehta, Roshni; Harris, David T.; Zack, Jerome A.; Ahmad, Nafees -.

In: Virology, Vol. 400, No. 1, 25.04.2010, p. 32-43.

Research output: Contribution to journalArticle

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