Differential expression of sodium pump catalytic subunits in the lens epithelium and fibers

N. A. Delamere, W. L. Dean, J. M. Stidam, A. E. Moseley

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

NaK-ATPase in lens epithelium plays a key role in conducting sodium-potassium transport. The purpose of this study was to test whether epithelium or fiber cells can synthesize new NaK-ATPase protein in response to an increase of membrane permeability. Western blot methodology was used to identify NaK-ATPase α subunit polypeptides in membrane material isolated from lens cells. As judged by immunoblot density, epithelial cell membrane material isolated from porcine lenses cultured 24 h with 1 μM amphotericin B contained more NaK-ATPase α subunit polypeptide than epithelial material isolated from control lenses. This increase stemmed from the apparent synthesis of NaK-ATPase α2 isoform polypeptide by the epithelium; NaK-ATPase αi isoform polypeptide abundance was not detectably altered. The apparent amphotericin B-induced expression of NaK-ATPase α2 was seen in lens epithelial cells but not fiber cells. This study suggests that the epithelium of the adult porcine lens may be capable of expressing additional sodium pump molecules of the α2-inftype when membrane permeability is increased.

Original languageEnglish (US)
Pages (from-to)73-76
Number of pages4
JournalOphthalmic Research
Volume28
Issue number1
DOIs
StatePublished - Jan 1 1996
Externally publishedYes

Keywords

  • Amphotericin B
  • Lens
  • Membrane permeability
  • NaK-ATPase

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Fingerprint Dive into the research topics of 'Differential expression of sodium pump catalytic subunits in the lens epithelium and fibers'. Together they form a unique fingerprint.

  • Cite this