Differential gene expression profiling of CD34+ CD133 + umbilical cord blood hematopoietic stem progenitor cells

Xianghui He, Veronica Gonzalez, Amy Tsang, Jennifer Thompson, Tom C. Tsang, David T. Harris

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Umbilical cord blood (CB)-derived primitive hematopoietic stem progenitor cells (HSPC) are a promising source for stem cell-based gene therapy due to the reduced incidence and severity of graft-versus-host disease (GVHD) after human leukocyte antigen (HLA)-disparate CB transplantation. Cell-surface markers such as CD34 and CD133 have been used in combination to enrich primitive HSPC for research and clinical applications. To understand the molecular characteristics of the CB HSPC, we compared the global gene expression of freshly isolated CB CD34+CD133+ cells with their progenies using a cDNA microarray containing 22,000 human cDNA clones printed on a single chip. A total of 139 genes were differentially expressed between CB HSPC and their progenies. These transcripts included a number of known genes that might play roles in key functions of CB HSPC as well as many genes of unknown function. Among the genes showing the greatest differential expression levels in HSPC were: psoriasin 1, CRHBP, HDAC3, MLLT3, HBEX2, SPINK2, c-kit, H2BFQ, CD133, HHEX, TCF4, ALDH1A1, and FHL1. These data provide more information on the molecular phenotype of CB HSPC and may lead to the identification of new genes critical to stem cell function.

Original languageEnglish (US)
Pages (from-to)188-198
Number of pages11
JournalStem Cells and Development
Volume14
Issue number2
DOIs
StatePublished - Apr 2005

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Gene Expression Profiling
Hematopoietic Stem Cells
Fetal Blood
Genes
Stem Cells
Stem Cell Research
Graft vs Host Disease
HLA Antigens
Oligonucleotide Array Sequence Analysis
Genetic Therapy
Complementary DNA
Clone Cells
Transplantation

ASJC Scopus subject areas

  • Hematology

Cite this

Differential gene expression profiling of CD34+ CD133 + umbilical cord blood hematopoietic stem progenitor cells. / He, Xianghui; Gonzalez, Veronica; Tsang, Amy; Thompson, Jennifer; Tsang, Tom C.; Harris, David T.

In: Stem Cells and Development, Vol. 14, No. 2, 04.2005, p. 188-198.

Research output: Contribution to journalArticle

He, Xianghui ; Gonzalez, Veronica ; Tsang, Amy ; Thompson, Jennifer ; Tsang, Tom C. ; Harris, David T. / Differential gene expression profiling of CD34+ CD133 + umbilical cord blood hematopoietic stem progenitor cells. In: Stem Cells and Development. 2005 ; Vol. 14, No. 2. pp. 188-198.
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