Differential glucocorticoid effects on stress-induced gene expression in the paraventricular nucleus of the hypothalamus and ACTH secretion in the rat

Thaddeus Wesley Warren Pace, Reginald I. Gaylord, Erin Jarvis, Milena Girotti, Robert L. Spencer

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Although previous studies have examined the extent to which adrenocorticotropic hormone (ACTH) secretion depends on endogenous glucocorticoid levels, few have examined the parallel glucocorticoid dependency of gene expression within the corticotropin releasing hormone (CRH) neuron containing subregion of the hypothalamic paraventricular nucleus (PVN). This study examined resting and stress-induced expression of three immediate early genes (c-fos, zif268, and NGFI-B mRNAs) and two phenotypic restricted immediate early genes that code for ACTH secretagogues (CRH and arginine vasopressin [AVP] hnRNAs) in the PVN of adrenalectomized (ADX) rats given either 0.9% saline to drink for 5 days or saline with corticosterone (CORT; 25 μg/ml). CORT-containing saline was replaced with saline 18 h before testing to ensure clearance of CORT at the time of testing. Dependent measures were examined 0, 15, 30, 60, or 120 min after 30 min restraint. Compared to sham surgery, ADX produced a large upregulation of basal ACTH secretion but only a trend for an increase in basal PVN CRH and parvocellular (mp) PVN AVP hnRNA expression, and a marked augmentation of restraint-induced ACTH secretion and the expression of all five genes examined. CORT containing saline partially normalized basal and restraint-induced ACTH secretion and restraint-induced AVP hnRNA, c-fos mRNA, and zif268 mRNA in the PVN in ADX rats. In contrast, expression patterns of restraint-induced PVN CRH hnRNA and NGFI-B mRNA were not different between ADX rats with or without CORT replacement. Given that there was no circulating CORT present at the time of restraint challenge in either group of ADX rats, the differential impact of CORT replacement on restraint-induced PVN gene expression must reflect differential dependency of the expression of these genes in the PVN on the prior presence of CORT.

Original languageEnglish (US)
Pages (from-to)400-411
Number of pages12
JournalStress
Volume12
Issue number5
DOIs
StatePublished - 2009
Externally publishedYes

Fingerprint

Paraventricular Hypothalamic Nucleus
Adrenocorticotropic Hormone
Glucocorticoids
Hypothalamus
Gene Expression
Corticotropin-Releasing Hormone
Arginine Vasopressin
Messenger RNA
Immediate-Early Genes
Corticosterone
Basal Ganglia
Up-Regulation
Neurons

Keywords

  • AVP
  • CRH
  • Glucocorticoid negative feedback
  • HPA axis
  • Immediate early gene
  • PVN

ASJC Scopus subject areas

  • Behavioral Neuroscience
  • Endocrine and Autonomic Systems
  • Physiology
  • Psychiatry and Mental health
  • Neuropsychology and Physiological Psychology

Cite this

Differential glucocorticoid effects on stress-induced gene expression in the paraventricular nucleus of the hypothalamus and ACTH secretion in the rat. / Pace, Thaddeus Wesley Warren; Gaylord, Reginald I.; Jarvis, Erin; Girotti, Milena; Spencer, Robert L.

In: Stress, Vol. 12, No. 5, 2009, p. 400-411.

Research output: Contribution to journalArticle

Pace, Thaddeus Wesley Warren ; Gaylord, Reginald I. ; Jarvis, Erin ; Girotti, Milena ; Spencer, Robert L. / Differential glucocorticoid effects on stress-induced gene expression in the paraventricular nucleus of the hypothalamus and ACTH secretion in the rat. In: Stress. 2009 ; Vol. 12, No. 5. pp. 400-411.
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