Differential localization and activity of the A- and B-forms of the human progesterone receptor using green fluorescent protein chimeras

Carol S. Lim, Christopher T. Baumann, Han Htun, Wenjuan Xian, Masako Irie, Catharine L. Smith, Gordon L. Hager

Research output: Contribution to journalArticlepeer-review

114 Scopus citations

Abstract

Subcellular localization and transcriptional activity of green fluorescent protein-progesterone receptor A and B chimeras (GFP-PRA and GFP- PRB) were examined in living mammalian cells. Both GFP-PRA and B chimeras were found to be similar in transcriptional activity compared with their non- GFP counterparts. GFP-PRA and PRA were both weakly active, while GFP-PRB and PRB gave a 20- to 40-fold induction using a reporter gene containing the full-length mouse mammary tumor virus long-terminal repeat linked to the luciferase gene (pLTRluc). Using fluorescence microscopy, nuclear/cytoplasmic distributions for the unliganded and hormone activated forms of GFP-PRA and GFP-PRB were characterized. The two forms of the receptor were found to have distinct intracellular distributions; GFP-PRA was found to be more nuclear than GFP-PRB in four cell lines examined. The causes for and implications of this differential localization of the A and B forms of the human PR are discussed.

Original languageEnglish (US)
Pages (from-to)366-375
Number of pages10
JournalMolecular Endocrinology
Volume13
Issue number3
DOIs
StatePublished - 1999

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology

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