Differential localization and activity of the A- and B-forms of the human progesterone receptor using green fluorescent protein chimeras

Carol S. Lim, Christopher T. Baumann, Han Htun, Wenjuan Xian, Masako Irie, Catharine Smith, Gordon L. Hager

Research output: Contribution to journalArticle

111 Citations (Scopus)

Abstract

Subcellular localization and transcriptional activity of green fluorescent protein-progesterone receptor A and B chimeras (GFP-PRA and GFP- PRB) were examined in living mammalian cells. Both GFP-PRA and B chimeras were found to be similar in transcriptional activity compared with their non- GFP counterparts. GFP-PRA and PRA were both weakly active, while GFP-PRB and PRB gave a 20- to 40-fold induction using a reporter gene containing the full-length mouse mammary tumor virus long-terminal repeat linked to the luciferase gene (pLTRluc). Using fluorescence microscopy, nuclear/cytoplasmic distributions for the unliganded and hormone activated forms of GFP-PRA and GFP-PRB were characterized. The two forms of the receptor were found to have distinct intracellular distributions; GFP-PRA was found to be more nuclear than GFP-PRB in four cell lines examined. The causes for and implications of this differential localization of the A and B forms of the human PR are discussed.

Original languageEnglish (US)
Pages (from-to)366-375
Number of pages10
JournalMolecular Endocrinology
Volume13
Issue number3
StatePublished - 1999
Externally publishedYes

Fingerprint

Progesterone Receptors
Green Fluorescent Proteins
Mouse mammary tumor virus
Terminal Repeat Sequences
Luciferases
Reporter Genes
Fluorescence Microscopy
Hormones
Cell Line
Genes
progesterone receptor A
progesterone receptor B

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology, Diabetes and Metabolism

Cite this

Differential localization and activity of the A- and B-forms of the human progesterone receptor using green fluorescent protein chimeras. / Lim, Carol S.; Baumann, Christopher T.; Htun, Han; Xian, Wenjuan; Irie, Masako; Smith, Catharine; Hager, Gordon L.

In: Molecular Endocrinology, Vol. 13, No. 3, 1999, p. 366-375.

Research output: Contribution to journalArticle

Lim, Carol S. ; Baumann, Christopher T. ; Htun, Han ; Xian, Wenjuan ; Irie, Masako ; Smith, Catharine ; Hager, Gordon L. / Differential localization and activity of the A- and B-forms of the human progesterone receptor using green fluorescent protein chimeras. In: Molecular Endocrinology. 1999 ; Vol. 13, No. 3. pp. 366-375.
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AU - Irie, Masako

AU - Smith, Catharine

AU - Hager, Gordon L.

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