Differential modulation of prohormone convertase mRNA by second messenger activators in two cholecystokinin-producing cell lines

Barbara L. Mania-Farnell, Ihab Botros, Robert Day, Thomas P. Davis

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Regulation of prohormone convertase expression was studied in two neuropeptide-producing cell lines, the human neuroepithelioma cell line SK-N-MCIXC and the rat medullary thyroid carcinoma cell line WE 4/2. The cells were treated with the phosphodiesterase inhibitor isobutyl-methylxanthine and the tumor-promoting phorbol ester, phorbol-12-myristate-13 acetate, activators of the cyclic AMP (cAMP) and protein kinase C (PKC) second messenger pathways, respectively. mRNA levels of prohormone convertase 1 (PC1), prohormone convertase 2 (PC2), and furin were determined after 3- and 6-h treatments, using Northern analysis. Activation of both cAMP and PKC pathways increased PC1, but not PC2 or furin mRNA levels in SK-N-MCIXC cells. Activation of the cAMP pathway increased PC1, PC2, and furin mRNA levels in WE 4/2 cells, whereas activation of the PKC pathway did not change prohormone convertase mRNA levels in this cell line. These results indicate that prohormone convertases may be differentially regulated by cAMP and PKC mechanisms and regulation may be tissue specific.

Original languageEnglish (US)
Pages (from-to)47-54
Number of pages8
JournalPeptides
Volume17
Issue number1
DOIs
StatePublished - 1996

Keywords

  • Cell lines
  • Gene regulation
  • PKC
  • Peptide regulation
  • Peptide synthesis
  • Prohormone convertase
  • Second messengers
  • cAMP

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

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