Differential potentiation of 1,3-bis(2-chloroethyl)-1-nitrosourea by α-difluoromethylornithine in chloroethylnitrosourea-sensitive and -resistant 91 rat brain tumor cells in vitro

Stina M. Oredsson, Philip J. Tofilon, Burt G. Feuerstein, Mark L. Rosenblum

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

α-Difluoromethylomithine, an enzyme-activated, irreversible inhibitor of omithine decarboxylase, inhibited the growth of both chloroethyl nitrosourea-sensitive and -esistant 9L rat brain tumor cells in vitro. After 48 hr of treatment with 10 mM α-difluoromethylomithine, the putrescine and spermidine contents of both resistant and sensitive cells were less than 5% of control levels, but the spermine levelwas slightly elevated. The cytotoxicity of 1,3-bis(2-chloroethyl)-1 -nitrosourea, as measured by a colony-forming efficiency assay, was significantly increased in α-difluoromethylomithine-pretreated sensitive cells but not in resistant cells treated with this polyamine inhibitor. With the sister chromatid exchange assay, we found thatα-difluoromethylornithine pretreatment increased 1,3-bis(2-chloroethyl)-1 -nitrosourea-induced damage to chromosomes in sensitive but not in resistant cells.

Original languageEnglish (US)
Pages (from-to)3576-3578
Number of pages3
JournalCancer Research
Volume43
Issue number8
StatePublished - Aug 1 1983
Externally publishedYes

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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