Differential structural requirements for the MSH and MCH activities of melanin concentrating hormone

Mac E. Hadley, Christian Zechel, Brain C. Wilkes, Ana M. Ana, Maria A. Visconti, Manuel Pozo-Alonso, Victor J Hruby

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Abstract

{A figure is presented} melanin concentrating hormone (MCH), exhibits both melanin granule concentrating and dispersing (MSH-like) activities. Fragment analogues of MCH were synthesized as described herein and the melanotropic activities of the peptides were determined. In the frog (Rana pipiens) and lizard (Anolis carolinensis) skin bioassays, the 5-17 and 5-14 fragments of MCH were inactive (at concentrations of 10-5M or less), whereas the 1-14 sequence exhibited minimal (about 10%) MSH-like activity compared to MCH, which, as reported previously, was about 600 times less active than α-MSH. In the teleost (fish) skin bioassay, the MCH5-17 analogue was equipotent to MCH, whereas the 1-14 analogue was 10-30 times and the cyclic N- and C- terminal truncated analogue, MCH5-14, was about 300 times less active than MCH. These results suggest that the N-terminal sequence is particularly critical to MSH-like activity in the tetrapod species studied, whereas other structural regions of MCH, particularly in the C-terminal, are more related to MCH activity in teleosts.

Original languageEnglish (US)
Pages (from-to)1139-1145
Number of pages7
JournalLife Sciences
Volume40
Issue number12
DOIs
Publication statusPublished - Mar 23 1987

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ASJC Scopus subject areas

  • Pharmacology

Cite this

Hadley, M. E., Zechel, C., Wilkes, B. C., Ana, A. M., Visconti, M. A., Pozo-Alonso, M., & Hruby, V. J. (1987). Differential structural requirements for the MSH and MCH activities of melanin concentrating hormone. Life Sciences, 40(12), 1139-1145. https://doi.org/10.1016/0024-3205(87)90231-1