Differentially expressed cDNAs in Alternaria alternata treated with 2-propenyl isothiocyanate

María Elena Báez-Flores, Rosalba Troncoso-Rojas, María A.Islas Osuna, Marisela Rivera Domínguez, Barry Pryor, Martín Ernesto Tiznado-Hernández

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

The molecular mechanism of the fungal tolerance phenotype to fungicides is not completely understood. This knowledge would allow for the development of environmentally friendly strategies for the control of fungal infection. With the goal of determining genes induced by 2p-ITC, a forward suppressive subtractive hybridization was performed using cDNAs from ITC-treated Alternaria alternata as a " tester" and from untreated fungus as a " driver." Using this approach, a library containing 102 ESTs was generated that resulted in 50 sequences after sequence assembly (17 contigs and 33 singletons). Blastx analysis revealed that 38% and 40% of the sequences showed significant similarity with known and hypothetical proteins, respectively, whereas 18% were not similar to known genes. These last sequences could represent novel genes that play an unknown role in the molecular responses of fungi during adaptation to 2p-ITC. Clones similar to opsins, ABC transporters, calmodulin, ATPases and SNOG proteins were identified. Using real-time RT-PCR analysis, significant inductions of an ABC transporter and a Ca ++ ATPase during 2p-ITC treatment were discovered. These results suggest that the fungal resistance phenotype to 2p-ITC involves calcium ions and 2p-ITC efflux via an ABC transporter.

Original languageEnglish (US)
Pages (from-to)566-577
Number of pages12
JournalMicrobiological Research
Volume166
Issue number7
DOIs
StatePublished - Oct 20 2011

Keywords

  • 2-Propenyl isothiocyanate
  • Alternaria alternata
  • Expressed sequence tags
  • Suppressive substractive hybridization

ASJC Scopus subject areas

  • Microbiology

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