Differentiating Proteomic Biomarkers in Breast Cancer by Laser Capture Microdissection and MALDI MS

Melinda E. Sanders, Eduardo C. Dias, Baogang J. Xu, James A. Mobley, David D Billheimer, Heinrich Roder, Julia Grigorieva, Mitchell Dowsett, Carlos L. Arteaga, Richard M. Caprioli

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

We assessed proteomic patterns in breast cancer using MALDI MS and laser capture microdissected cells. Protein and peptide expression in invasive mammary carcinoma versus normal mammary epithelium and estrogen-receptor positive versus estrogen-receptor negative tumors were compared. Biomarker candidates were identified by statistical analysis and classifiers were developed and validated in blinded test sets. Several of the mlz features used in the classifiers were identified by LC-MS/MS and two were confirmed by immunohistochemistry.

Original languageEnglish (US)
Pages (from-to)1500-1507
Number of pages8
JournalJournal of Proteome Research
Volume7
Issue number4
DOIs
StatePublished - Apr 2008
Externally publishedYes

Fingerprint

Microdissection
Laser Capture Microdissection
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Biomarkers
Estrogen Receptors
Proteomics
Classifiers
Breast Neoplasms
Lasers
Tumors
Statistical methods
Breast
Epithelium
Immunohistochemistry
Peptides
Neoplasms
Proteins

Keywords

  • Breast cancer
  • Laser capture microdissection
  • MALDI MS

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)
  • Genetics
  • Biotechnology

Cite this

Differentiating Proteomic Biomarkers in Breast Cancer by Laser Capture Microdissection and MALDI MS. / Sanders, Melinda E.; Dias, Eduardo C.; Xu, Baogang J.; Mobley, James A.; Billheimer, David D; Roder, Heinrich; Grigorieva, Julia; Dowsett, Mitchell; Arteaga, Carlos L.; Caprioli, Richard M.

In: Journal of Proteome Research, Vol. 7, No. 4, 04.2008, p. 1500-1507.

Research output: Contribution to journalArticle

Sanders, ME, Dias, EC, Xu, BJ, Mobley, JA, Billheimer, DD, Roder, H, Grigorieva, J, Dowsett, M, Arteaga, CL & Caprioli, RM 2008, 'Differentiating Proteomic Biomarkers in Breast Cancer by Laser Capture Microdissection and MALDI MS', Journal of Proteome Research, vol. 7, no. 4, pp. 1500-1507. https://doi.org/10.1021/pr7008109
Sanders, Melinda E. ; Dias, Eduardo C. ; Xu, Baogang J. ; Mobley, James A. ; Billheimer, David D ; Roder, Heinrich ; Grigorieva, Julia ; Dowsett, Mitchell ; Arteaga, Carlos L. ; Caprioli, Richard M. / Differentiating Proteomic Biomarkers in Breast Cancer by Laser Capture Microdissection and MALDI MS. In: Journal of Proteome Research. 2008 ; Vol. 7, No. 4. pp. 1500-1507.
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