Differentiation of BP-type baculovirus strains using in situ hybridization

S. Durand, D. V. Lightner, J. R. Bonami

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

BP-type viruses infect wild and famed shrimp species in the Americas and can cause high mortality in larval stages. Histologically, BP disease is characterized by the presence of occlusion bodies (OBs) in the nucleus of hepatopancreatic epithelial cells. By in situ hybridization using different molecular probes, we tested shrimp infected with BP-type viruses collected from several geographical areas. In one shrimp, a case of double infection by 2 different BP-type viruses was noted. This has not been previously reported. The molecular probes recognized only the PAcific strain of BP. This specificity of the probe was confirmed by in situ hybridization tests with some infected shrimp collected from the Atlantic and Pacific coasts. The probes reacted only with infected shrimp obtained from the Pacific coast. These results suggest the existence of at least 2 different BP-type viruses and show that specific probes can be used to differentiate between them.

Original languageEnglish (US)
Pages (from-to)237-239
Number of pages3
JournalDiseases of aquatic organisms
Volume32
Issue number3
DOIs
StatePublished - Apr 3 1998

Keywords

  • BP
  • Baculovirus
  • In situ hybridization
  • Non-radioactive probes
  • Shrimp

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Aquatic Science

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