Diffuse spectral fundus reflectance measured using subretinally placed spectralon

David A. Salyer, Kurt R Denninghoff, Neil Beaudry, Sreenivasa Basavanthappa, Robert I. Park, Russell A Chipman

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The diffuse fundus reflectance and the spectral transmittance of the swine sensory retina was measured in vivo using intravitreal illumination. Pars plana vitrectomy and intravitreal manipulations were performed on a female American Yorkshire domestic swine. Light from a scanning monochromator was coupled into a fiber optic intraocular illuminator inserted into the vitreous. A 1.93-mm 2 region of the illuminated fundus was imaged from an oblique illumination angle. Multispectral retinal images were acquired for four experimental conditions: the eye (1) prior to vitrectomy, (2) after vitrectomy, (3) after insertion of a Spectralon disk super-retinally, and (4) after subretinal insertion of the disk. The absorption of melanin and hemoglobin in the red wavelengths was used to convert relative spectral reflectance to absolute reflectance. The flux scattered from the super-retinal Spectralon was used to correct for scattering in the globe. The transmittance of the sensory retina was measured in vivo using the scatter corrected subretinal Spectralon disk reflectance. The hemoglobin and melanin components of the spectrum due to scattered light were removed from the retinal transmission spectrum. The in vivo spectral transmittance of the sensory retina in this swine was essentially flat across the visible spectrum, with an average transmittance >90%.

Original languageEnglish (US)
Article number044004
JournalJournal of Biomedical Optics
Volume13
Issue number4
DOIs
StatePublished - 2008

Fingerprint

spectral reflectance
swine
retina
transmittance
Melanin
melanin
Hemoglobin
Melanins
hemoglobin
reflectance
insertion
Hemoglobins
Lighting
illumination
retinal images
illuminators
globes
Temazepam
Monochromators
monochromators

Keywords

  • ophthalmology
  • reflectance
  • spectroscopy

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics

Cite this

Diffuse spectral fundus reflectance measured using subretinally placed spectralon. / Salyer, David A.; Denninghoff, Kurt R; Beaudry, Neil; Basavanthappa, Sreenivasa; Park, Robert I.; Chipman, Russell A.

In: Journal of Biomedical Optics, Vol. 13, No. 4, 044004, 2008.

Research output: Contribution to journalArticle

Salyer, David A. ; Denninghoff, Kurt R ; Beaudry, Neil ; Basavanthappa, Sreenivasa ; Park, Robert I. ; Chipman, Russell A. / Diffuse spectral fundus reflectance measured using subretinally placed spectralon. In: Journal of Biomedical Optics. 2008 ; Vol. 13, No. 4.
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