Diffusion-ordered nuclear magnetic resonance spectroscopy for analysis of DNA secondary structural elements

Attila Ambrus, Danzhou Yang

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

Structure determination of secondary DNA structural elements, such as G-quadruplexes, gains an increasing importance as fundamental physiological roles are being associated with the formation of such structures in vivo. A truncated native DNA sequence generally requires further optimization to obtain a candidate with desired nuclear magnetic resonance (NMR) properties for structural analysis in solution. The optimum sequence is expected to form one dominant, stable molecular entity in solution with well-resolved NMR peaks. However, DNA sequences are prone to form structures composed of one, two, three, or four strands depending on sequence and solution conditions. The thorough characterization of the molecularity (stoichiometry and molecular weight) and appropriate solution conditions for sequences with different modifications traditionally applies analytical techniques that generally do not represent the solution conditions for NMR structure determination. Here we present the application of diffusion-ordered NMR spectroscopy as a useful analytical tool for the optimization and analysis of DNA secondary structural elements, specifically, the DNA G-quadruplex structures, including those formed in the human telomeric sequence and in the promoter regions of bcl-2 and c-myc genes.

Original languageEnglish (US)
Pages (from-to)56-67
Number of pages12
JournalAnalytical Biochemistry
Volume367
Issue number1
DOIs
StatePublished - Aug 1 2007

Keywords

  • DNA
  • DOSY
  • Human telomere
  • NMR
  • Quadruplex
  • bcl-2
  • c-myc

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Diffusion-ordered nuclear magnetic resonance spectroscopy for analysis of DNA secondary structural elements'. Together they form a unique fingerprint.

  • Cite this