Digital expression profiling of a grape-bud EST collection leads to new insight into molecular events during grape-bud dormancy release

Tsvika Keilin, Xuequn Pang, Jaganatha Venkateswari, Tamar Halaly, Omer Crane, Alexandra Keren, Aliza Ogrodovitch, Ron Ophir, Hanne Volpin, David Galbraith, Etti Or

Research output: Contribution to journalArticle

37 Scopus citations

Abstract

The application of genomic approaches may serve as an initial step in broadening our understanding of the complex network of biochemical and cellular processes responsible for the regulation and execution of grape-bud dormancy release. However, bud tissue in general, and the dormant bud in particular, are under-represented in the public Vitis genomic resources. Here we describe a large-scale grape-bud EST collection representing a wide range of bud developmental stages. A collection of 5516 consensus sequences is presented, of which 59% were not included within the Vitis TIGR collection at the time of current analysis. About 22% of these transcripts bear no resemblance to any known plant transcript and thus corroborate the need for this targeted EST collection. The added value of the presented EST collection lies in the conferred ability to compare EST frequencies between the different cDNA libraries. Such comparison was implemented and allowed us to identify several genes/functions whose expression is altered in response to the dormancy-release treatment. Based on this analysis, it is suggested that oxidative stress, calcium signaling, intracellular vesicle trafficking and anaerobic mode of carbohydrate metabolism play a role in the regulation and execution of grape-bud dormancy release.

Original languageEnglish (US)
Pages (from-to)446-457
Number of pages12
JournalPlant Science
Volume173
Issue number4
DOIs
StatePublished - Oct 1 2007

Keywords

  • Bud dormancy
  • Carbohydrate metabolism
  • Expressed sequence tag (EST)
  • Oxidative stress
  • Vesicle trafficking
  • Vitis

ASJC Scopus subject areas

  • Genetics
  • Agronomy and Crop Science
  • Plant Science

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    Keilin, T., Pang, X., Venkateswari, J., Halaly, T., Crane, O., Keren, A., Ogrodovitch, A., Ophir, R., Volpin, H., Galbraith, D., & Or, E. (2007). Digital expression profiling of a grape-bud EST collection leads to new insight into molecular events during grape-bud dormancy release. Plant Science, 173(4), 446-457. https://doi.org/10.1016/j.plantsci.2007.07.004