Dioldehydratase binds coenzyme B12 in the 'base-on' mode: ESR investigations on Cob(II)alamin

Andreas Abend, Rainer Nitsche, Vahe Bandarian, Erhard Stupperich, János Rétey

Research output: Contribution to journalArticle

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Abstract

Even in the enzyme-bound state the dimethyl-benzimidazole ligand in the dioldehydratase from Salmonella typhimurium remains bound to the cobalt ion in contrast to some coenzyme B12- dependent enzymes. Direct, ESR spectroscopic proof for this 'base-on' binding mode was obtained by using a coenzyme in which one of the nitrogen atoms of the dimethylbenzimidazole ligand was 15N labeled.

Original languageEnglish (US)
Pages (from-to)625-627
Number of pages3
JournalAngewandte Chemie - International Edition
Volume37
Issue number5
DOIs
StatePublished - Mar 16 1998

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Keywords

  • Bioorganic chemistry
  • Coenzymes
  • EPR spectroscopy
  • Isotopic labeling
  • Vitamin B

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

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