Direct demonstration of instabilities in oxygen concentrations within the extravascular compartment of an experimental tumor

Jennifer Lanzen, Rod D. Braun, Bruce Klitzman, David Brizel, Timothy W. Secomb, Mark W. Dewhirst

Research output: Contribution to journalArticle

98 Scopus citations

Abstract

To test the hypothesis that temporal variations in microvessel red cell flux cause unstable oxygen levels in tumor interstitium, extravascular oxygenation of R3230Ac mammary tumors grown in skin-fold window chambers was measured using recessed tip polarographic microelectrodes. Red cell fluxes in microvessels surrounding pO2 measurement locations were measured using fluorescently labeled red cells. Temporal pO2 instability was observed in all experiments. Median pO2 was inversely related to radial distance from microvessels. Transient fluctuations above and below 10 mm Hg were consistently seen, except in one experiment near the oxygen diffusion distance limit (140 μm) where pO2 fluctuations were <2 mm Hg and median pO2 was <5 mm Hg. Vascular stasis was not seen in these experiments. These results show that fluctuations in red cell flux, as opposed to vascular stasis, can cause temporal variations in pO2 that extend from perivascular regions to the maximum oxygen diffusion distance.

Original languageEnglish (US)
Pages (from-to)2219-2223
Number of pages5
JournalCancer Research
Volume66
Issue number4
DOIs
StatePublished - Feb 15 2006

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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