Abstract
Methodologies to detect DNA sequences with high sensitivity and specificity have tremendous potential as molecular diagnostic agents. Most current methods exploit the ability of single-stranded DNA (ssDNA) to base pair with high specificity to a complementary molecule. However, recent advances in robust techniques for recognition of DNA in the major and minor groove have made possible the direct detection of double-stranded DNA (dsDNA), without the need for denaturation, renaturation, or hybridization. This review will describe the progress in adapting polyamides, triplex DNA, and engineered zinc finger DNA-binding proteins as dsDNA diagnostic systems. In particular, the sequence-enabled reassembly (SEER) method, involving the use of custom zinc finger proteins, offers the potential for direct detection of dsDNA in cells, with implications for cell-based diagnostics and therapeutics.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 551-560 |
| Number of pages | 10 |
| Journal | Molecular BioSystems |
| Volume | 2 |
| Issue number | 11 |
| DOIs | |
| State | Published - 2006 |
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ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology (miscellaneous)
Cite this
Direct detection of double-stranded DNA : Molecular methods and applications for DNA diagnostics. / Ghosh, Indraneel; Stains, Cliff I.; Ooi, Aik T.; Segal, David J.
In: Molecular BioSystems, Vol. 2, No. 11, 2006, p. 551-560.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Direct detection of double-stranded DNA
T2 - Molecular methods and applications for DNA diagnostics
AU - Ghosh, Indraneel
AU - Stains, Cliff I.
AU - Ooi, Aik T.
AU - Segal, David J.
PY - 2006
Y1 - 2006
N2 - Methodologies to detect DNA sequences with high sensitivity and specificity have tremendous potential as molecular diagnostic agents. Most current methods exploit the ability of single-stranded DNA (ssDNA) to base pair with high specificity to a complementary molecule. However, recent advances in robust techniques for recognition of DNA in the major and minor groove have made possible the direct detection of double-stranded DNA (dsDNA), without the need for denaturation, renaturation, or hybridization. This review will describe the progress in adapting polyamides, triplex DNA, and engineered zinc finger DNA-binding proteins as dsDNA diagnostic systems. In particular, the sequence-enabled reassembly (SEER) method, involving the use of custom zinc finger proteins, offers the potential for direct detection of dsDNA in cells, with implications for cell-based diagnostics and therapeutics.
AB - Methodologies to detect DNA sequences with high sensitivity and specificity have tremendous potential as molecular diagnostic agents. Most current methods exploit the ability of single-stranded DNA (ssDNA) to base pair with high specificity to a complementary molecule. However, recent advances in robust techniques for recognition of DNA in the major and minor groove have made possible the direct detection of double-stranded DNA (dsDNA), without the need for denaturation, renaturation, or hybridization. This review will describe the progress in adapting polyamides, triplex DNA, and engineered zinc finger DNA-binding proteins as dsDNA diagnostic systems. In particular, the sequence-enabled reassembly (SEER) method, involving the use of custom zinc finger proteins, offers the potential for direct detection of dsDNA in cells, with implications for cell-based diagnostics and therapeutics.
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U2 - 10.1039/b611169f
DO - 10.1039/b611169f
M3 - Article
VL - 2
SP - 551
EP - 560
JO - Molecular BioSystems
JF - Molecular BioSystems
SN - 1742-206X
IS - 11
ER -