Direct detection of plasmid-mediated antibiotic resistance in bloodstream infection by PCR using wire-guided droplet manipulation (WDM)

Dustin K. Harshman, Roberto Reyes, Jeong Yeol Yoon

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Bloodstream infection (BSI), an expensive and deadly condition, burdens healthcare. Improved BSI treatment relies on rapid, point-of-care diagnosis of causative species and presence of plasmid-mediated drug resistance. We have designed, fabricated and assembled an apparatus for automated PCR amplification by wire-guided droplet manipulation (WDM). WDM minimizes thermal resistance and exploits convective heat transfer to increase reaction speeds. Molecular partitioning within the water-in-oil droplet enables PCR amplification with whole blood (WB) in situ. The V3 region of the 16s rRNA gene and a representative 4.884 kb plasmid were amplified by WDM from 10% WB. Optics has been designed for real-time detection.

Original languageEnglish (US)
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages470-472
Number of pages3
ISBN (Print)9781632666246
StatePublished - Jan 1 2013
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
Duration: Oct 27 2013Oct 31 2013

Publication series

Name17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Volume1

Other

Other17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
CountryGermany
CityFreiburg
Period10/27/1310/31/13

Keywords

  • 16s rRNA
  • Bloodstream infection
  • Molecular partitioning
  • PCR

ASJC Scopus subject areas

  • Bioengineering

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