Direct detection of plasmid-mediated antibiotic resistance in bloodstream infection by PCR using wire-guided droplet manipulation (WDM)

Dustin K. Harshman, Roberto Reyes, Jeong-Yeol Yoon

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Bloodstream infection (BSI), an expensive and deadly condition, burdens healthcare. Improved BSI treatment relies on rapid, point-of-care diagnosis of causative species and presence of plasmid-mediated drug resistance. We have designed, fabricated and assembled an apparatus for automated PCR amplification by wire-guided droplet manipulation (WDM). WDM minimizes thermal resistance and exploits convective heat transfer to increase reaction speeds. Molecular partitioning within the water-in-oil droplet enables PCR amplification with whole blood (WB) in situ. The V3 region of the 16s rRNA gene and a representative 4.884 kb plasmid were amplified by WDM from 10% WB. Optics has been designed for real-time detection.

Original languageEnglish (US)
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages470-472
Number of pages3
Volume1
ISBN (Print)9781632666246
StatePublished - 2013
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
Duration: Oct 27 2013Oct 31 2013

Other

Other17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
CountryGermany
CityFreiburg
Period10/27/1310/31/13

Fingerprint

Antibiotics
Plasmids
Wire
Anti-Bacterial Agents
Amplification
Blood
Heat resistance
Optics
Oils
Genes
Heat transfer
Water
Pharmaceutical Preparations

Keywords

  • 16s rRNA
  • Bloodstream infection
  • Molecular partitioning
  • PCR

ASJC Scopus subject areas

  • Bioengineering

Cite this

Harshman, D. K., Reyes, R., & Yoon, J-Y. (2013). Direct detection of plasmid-mediated antibiotic resistance in bloodstream infection by PCR using wire-guided droplet manipulation (WDM). In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 (Vol. 1, pp. 470-472). Chemical and Biological Microsystems Society.

Direct detection of plasmid-mediated antibiotic resistance in bloodstream infection by PCR using wire-guided droplet manipulation (WDM). / Harshman, Dustin K.; Reyes, Roberto; Yoon, Jeong-Yeol.

17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Vol. 1 Chemical and Biological Microsystems Society, 2013. p. 470-472.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Harshman, DK, Reyes, R & Yoon, J-Y 2013, Direct detection of plasmid-mediated antibiotic resistance in bloodstream infection by PCR using wire-guided droplet manipulation (WDM). in 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. vol. 1, Chemical and Biological Microsystems Society, pp. 470-472, 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, Freiburg, Germany, 10/27/13.
Harshman DK, Reyes R, Yoon J-Y. Direct detection of plasmid-mediated antibiotic resistance in bloodstream infection by PCR using wire-guided droplet manipulation (WDM). In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Vol. 1. Chemical and Biological Microsystems Society. 2013. p. 470-472
Harshman, Dustin K. ; Reyes, Roberto ; Yoon, Jeong-Yeol. / Direct detection of plasmid-mediated antibiotic resistance in bloodstream infection by PCR using wire-guided droplet manipulation (WDM). 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Vol. 1 Chemical and Biological Microsystems Society, 2013. pp. 470-472
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