Direct loading of polymer matrices in plastic microchips for rapid DNA analysis: A comparative study

Cedric Hurth, Jian Gu, Maurice Aboud, Matthew D. Estes, Alan R. Nordquist, Bruce McCord, Frederic Zenhausern

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We report the design and performance validation of microfluidic separation technologies for human identification using a disposable plastic device suitable for integration into an automated rapid DNA analysis system. A fabrication process for a 15-cm long hot-embossed plastic microfluidic devices with a smooth semielliptical cross section out of cyclic olefin copolymer is presented. We propose a mixed polymer solution of 95% w/v hydroxyethylcellulose and 5% w/v polyvinylpyrrolidone for a final polymer concentration of 2.5 or 3.0% to be used as coating and sieving matrix for DNA separation. This formulation allows preparing the microchip without pretreatment in a single-loading step and provides high-resolution separation (≈1.2 bp for fragments <200 bp), which is superior to existing commercial matrices under the same conditions. The hot-embossed device performance is characterized and compared to injection-molded devices made out of cyclic olefin copolymer based on their respective injector geometry, channel shape, and surface charges. Each device design is assessed by fluorescence videomicroscopy to evaluate the formation of injection plugs, then by comparing electropherograms for the separation of a DNA size standard relevant to human identification.

Original languageEnglish (US)
Pages (from-to)2604-2611
Number of pages8
JournalElectrophoresis
Volume33
Issue number16
DOIs
StatePublished - Aug 2012

Fingerprint

Oligonucleotide Array Sequence Analysis
Cycloparaffins
Polymer matrix
Plastics
Forensic Anthropology
Polymers
Lab-On-A-Chip Devices
Equipment and Supplies
DNA
Microfluidics
Equipment Design
Povidone
Video Microscopy
Injections
Copolymers
Polymer solutions
Surface charge
Fluorescence
Technology
Fabrication

Keywords

  • DNA separation
  • Microfluidics
  • Plastic microdevices

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

Cite this

Direct loading of polymer matrices in plastic microchips for rapid DNA analysis : A comparative study. / Hurth, Cedric; Gu, Jian; Aboud, Maurice; Estes, Matthew D.; Nordquist, Alan R.; McCord, Bruce; Zenhausern, Frederic.

In: Electrophoresis, Vol. 33, No. 16, 08.2012, p. 2604-2611.

Research output: Contribution to journalArticle

Hurth, Cedric ; Gu, Jian ; Aboud, Maurice ; Estes, Matthew D. ; Nordquist, Alan R. ; McCord, Bruce ; Zenhausern, Frederic. / Direct loading of polymer matrices in plastic microchips for rapid DNA analysis : A comparative study. In: Electrophoresis. 2012 ; Vol. 33, No. 16. pp. 2604-2611.
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