Direct transfer of antigen-specific cytolytic activity to noncytolytic cells upon fusion with liposomes derived from cytolytic T cell clones

David T. Harris, H. R. MacDonald, J. C. Gerottini

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Murine cytolytic T lymphocytes (CTL) clones were solubilized in Nonidet P-40 detergent, and the matrix and membrane proteins separated from the nuclear constituents. These proteins, in combination with exogenous lipids and Sendai virus envelope proteins, were used to construct liposomes that were then fused with noncytolytic cloned T cell recipients. The resultant fusion products were found to be highly cytolytic and appeared to express the same specificity as the original donor clone. Further analysis showed that the liposomal transfer process was extremely efficient. Moreover, in addition to noncytolytic T cell clones, three transformed T cell lines and one B cell line were found to express specific cytolytic activity after fusion with appropriate liposomes. Inhibition experiments using monoclonal antibodies against target cell antigens, as well as analysis of the lytic reactivity pattern of the fusion products, confirmed the high degree of specificity conferred to the recipient cells. This study thus indicates that the two characteristic typically associated with CTL, namely antigen-specific recognition and cytolytic activity, can be solubilized from CTL and transferred to recipient cells that do not express these characteristics.

Original languageEnglish (US)
Pages (from-to)261-275
Number of pages15
JournalJournal of Experimental Medicine
Volume159
Issue number1
StatePublished - 1984
Externally publishedYes

Fingerprint

Cell Fusion
Liposomes
Clone Cells
T-Lymphocytes
Antigens
Viral Envelope Proteins
Sendai virus
Transformed Cell Line
Detergents
Membrane Proteins
B-Lymphocytes
Monoclonal Antibodies
Lipids
Cell Line
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Direct transfer of antigen-specific cytolytic activity to noncytolytic cells upon fusion with liposomes derived from cytolytic T cell clones. / Harris, David T.; MacDonald, H. R.; Gerottini, J. C.

In: Journal of Experimental Medicine, Vol. 159, No. 1, 1984, p. 261-275.

Research output: Contribution to journalArticle

@article{37b356b07fd54305b7b25b47f9d66e35,
title = "Direct transfer of antigen-specific cytolytic activity to noncytolytic cells upon fusion with liposomes derived from cytolytic T cell clones",
abstract = "Murine cytolytic T lymphocytes (CTL) clones were solubilized in Nonidet P-40 detergent, and the matrix and membrane proteins separated from the nuclear constituents. These proteins, in combination with exogenous lipids and Sendai virus envelope proteins, were used to construct liposomes that were then fused with noncytolytic cloned T cell recipients. The resultant fusion products were found to be highly cytolytic and appeared to express the same specificity as the original donor clone. Further analysis showed that the liposomal transfer process was extremely efficient. Moreover, in addition to noncytolytic T cell clones, three transformed T cell lines and one B cell line were found to express specific cytolytic activity after fusion with appropriate liposomes. Inhibition experiments using monoclonal antibodies against target cell antigens, as well as analysis of the lytic reactivity pattern of the fusion products, confirmed the high degree of specificity conferred to the recipient cells. This study thus indicates that the two characteristic typically associated with CTL, namely antigen-specific recognition and cytolytic activity, can be solubilized from CTL and transferred to recipient cells that do not express these characteristics.",
author = "Harris, {David T.} and MacDonald, {H. R.} and Gerottini, {J. C.}",
year = "1984",
language = "English (US)",
volume = "159",
pages = "261--275",
journal = "Journal of Experimental Medicine",
issn = "0022-1007",
publisher = "Rockefeller University Press",
number = "1",

}

TY - JOUR

T1 - Direct transfer of antigen-specific cytolytic activity to noncytolytic cells upon fusion with liposomes derived from cytolytic T cell clones

AU - Harris, David T.

AU - MacDonald, H. R.

AU - Gerottini, J. C.

PY - 1984

Y1 - 1984

N2 - Murine cytolytic T lymphocytes (CTL) clones were solubilized in Nonidet P-40 detergent, and the matrix and membrane proteins separated from the nuclear constituents. These proteins, in combination with exogenous lipids and Sendai virus envelope proteins, were used to construct liposomes that were then fused with noncytolytic cloned T cell recipients. The resultant fusion products were found to be highly cytolytic and appeared to express the same specificity as the original donor clone. Further analysis showed that the liposomal transfer process was extremely efficient. Moreover, in addition to noncytolytic T cell clones, three transformed T cell lines and one B cell line were found to express specific cytolytic activity after fusion with appropriate liposomes. Inhibition experiments using monoclonal antibodies against target cell antigens, as well as analysis of the lytic reactivity pattern of the fusion products, confirmed the high degree of specificity conferred to the recipient cells. This study thus indicates that the two characteristic typically associated with CTL, namely antigen-specific recognition and cytolytic activity, can be solubilized from CTL and transferred to recipient cells that do not express these characteristics.

AB - Murine cytolytic T lymphocytes (CTL) clones were solubilized in Nonidet P-40 detergent, and the matrix and membrane proteins separated from the nuclear constituents. These proteins, in combination with exogenous lipids and Sendai virus envelope proteins, were used to construct liposomes that were then fused with noncytolytic cloned T cell recipients. The resultant fusion products were found to be highly cytolytic and appeared to express the same specificity as the original donor clone. Further analysis showed that the liposomal transfer process was extremely efficient. Moreover, in addition to noncytolytic T cell clones, three transformed T cell lines and one B cell line were found to express specific cytolytic activity after fusion with appropriate liposomes. Inhibition experiments using monoclonal antibodies against target cell antigens, as well as analysis of the lytic reactivity pattern of the fusion products, confirmed the high degree of specificity conferred to the recipient cells. This study thus indicates that the two characteristic typically associated with CTL, namely antigen-specific recognition and cytolytic activity, can be solubilized from CTL and transferred to recipient cells that do not express these characteristics.

UR - http://www.scopus.com/inward/record.url?scp=0021330211&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021330211&partnerID=8YFLogxK

M3 - Article

VL - 159

SP - 261

EP - 275

JO - Journal of Experimental Medicine

JF - Journal of Experimental Medicine

SN - 0022-1007

IS - 1

ER -