Directly measuring adhesive and elastic properties of bacteria using a surface force apparatus

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

Bacteria are increasingly being employed as components in biosensors and biofilm reactors. It is important to understand the material properties of bacteria in dry conditions for these applications. For a decade, Atomic Force Microscopy (AFM) has been the primary tool used to study the adhesion and elastic properties of individual bacteria. In this work we show it is possible to use a Surface Forces Apparatus (SFA) to measure elastic and adhesive properties of small collections of surface bound bacteria. The measurements are conducted with submonolayer, patterned bacterial films and we have developed a protocol to image the contact area with AFM after the experiment. Using the SFA, we measured the force profile between a Pseudomonas aeruginosa PAO1 film and a bare mica surface. Pseudomonas aeruginosa PAO1 is a ubiquitous gram-negative soil bacterium and is also an opportunistic pathogen. We repeated the measurement in the same contact position for a number of days to determine the effect of desiccation on the film material properties.

Original languageEnglish (US)
Title of host publicationACS Symposium Series
PublisherAmerican Chemical Society
Pages217-229
Number of pages13
Volume984
ISBN (Print)9780841274303
DOIs
StatePublished - May 30 2008

Publication series

NameACS Symposium Series
Volume984
ISSN (Print)00976156
ISSN (Electronic)19475918

Fingerprint

Adhesives
Bacteria
Atomic force microscopy
Materials properties
Mica
Biofilms
Pathogens
Biosensors
Adhesion
Soils
Experiments

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

Curry, J. E., Heo, C. H., & Maier, R. M. (2008). Directly measuring adhesive and elastic properties of bacteria using a surface force apparatus. In ACS Symposium Series (Vol. 984, pp. 217-229). (ACS Symposium Series; Vol. 984). American Chemical Society. https://doi.org/10.1021/bk-2008-0984.ch013

Directly measuring adhesive and elastic properties of bacteria using a surface force apparatus. / Curry, Joan E; Heo, Cheol Ho; Maier, Raina Margaret.

ACS Symposium Series. Vol. 984 American Chemical Society, 2008. p. 217-229 (ACS Symposium Series; Vol. 984).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Curry, JE, Heo, CH & Maier, RM 2008, Directly measuring adhesive and elastic properties of bacteria using a surface force apparatus. in ACS Symposium Series. vol. 984, ACS Symposium Series, vol. 984, American Chemical Society, pp. 217-229. https://doi.org/10.1021/bk-2008-0984.ch013
Curry JE, Heo CH, Maier RM. Directly measuring adhesive and elastic properties of bacteria using a surface force apparatus. In ACS Symposium Series. Vol. 984. American Chemical Society. 2008. p. 217-229. (ACS Symposium Series). https://doi.org/10.1021/bk-2008-0984.ch013
Curry, Joan E ; Heo, Cheol Ho ; Maier, Raina Margaret. / Directly measuring adhesive and elastic properties of bacteria using a surface force apparatus. ACS Symposium Series. Vol. 984 American Chemical Society, 2008. pp. 217-229 (ACS Symposium Series).
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