Discovery of a novel methanogen prevalent in thawing permafrost

Rhiannon Mondav, Ben J. Woodcroft, Eun Hae Kim, Carmody K. Mccalley, Suzanne B. Hodgkins, Patrick M. Crill, Jeffrey Chanton, Gregory B. Hurst, Nathan C. Verberkmoes, Scott Saleska, Philip Hugenholtz, Virginia I Rich, Gene W. Tyson

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

Thawing permafrost promotes microbial degradation of cryo-sequestered and new carbon leading to the biogenic production of methane, creating a positive feedback to climate change. Here we determine microbial community composition along a permafrost thaw gradient in northern Sweden. Partially thawed sites were frequently dominated by a single archaeal phylotype, Candidatus 'Methanoflorens stordalenmirensis' gen. nov. sp. nov., belonging to the uncultivated lineage 'Rice Cluster II' (Candidatus 'Methanoflorentaceae' fam. nov.). Metagenomic sequencing led to the recovery of its near-complete genome, revealing the genes necessary for hydrogenotrophic methanogenesis. These genes are highly expressed and methane carbon isotope data are consistent with hydrogenotrophic production of methane in the partially thawed site. In addition to permafrost wetlands, 'Methanoflorentaceae' are widespread in high methane-flux habitats suggesting that this lineage is both prevalent and a major contributor to global methane production. In thawing permafrost, Candidatus 'M. stordalenmirensis' appears to be a key mediator of methane-based positive feedback to climate warming.

Original languageEnglish (US)
Article number3212
JournalNature Communications
Volume5
DOIs
StatePublished - Feb 14 2014

Fingerprint

Methanogens
permafrost
Permafrost
Thawing
Methane
methane
melting
positive feedback
Genes
genes
Carbon Isotopes
wetlands
Feedback
Metagenomics
carbon isotopes
sequencing
habitats
Wetlands
Sweden
genome

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry(all)
  • Physics and Astronomy(all)

Cite this

Mondav, R., Woodcroft, B. J., Kim, E. H., Mccalley, C. K., Hodgkins, S. B., Crill, P. M., ... Tyson, G. W. (2014). Discovery of a novel methanogen prevalent in thawing permafrost. Nature Communications, 5, [3212]. https://doi.org/10.1038/ncomms4212

Discovery of a novel methanogen prevalent in thawing permafrost. / Mondav, Rhiannon; Woodcroft, Ben J.; Kim, Eun Hae; Mccalley, Carmody K.; Hodgkins, Suzanne B.; Crill, Patrick M.; Chanton, Jeffrey; Hurst, Gregory B.; Verberkmoes, Nathan C.; Saleska, Scott; Hugenholtz, Philip; Rich, Virginia I; Tyson, Gene W.

In: Nature Communications, Vol. 5, 3212, 14.02.2014.

Research output: Contribution to journalArticle

Mondav, R, Woodcroft, BJ, Kim, EH, Mccalley, CK, Hodgkins, SB, Crill, PM, Chanton, J, Hurst, GB, Verberkmoes, NC, Saleska, S, Hugenholtz, P, Rich, VI & Tyson, GW 2014, 'Discovery of a novel methanogen prevalent in thawing permafrost', Nature Communications, vol. 5, 3212. https://doi.org/10.1038/ncomms4212
Mondav R, Woodcroft BJ, Kim EH, Mccalley CK, Hodgkins SB, Crill PM et al. Discovery of a novel methanogen prevalent in thawing permafrost. Nature Communications. 2014 Feb 14;5. 3212. https://doi.org/10.1038/ncomms4212
Mondav, Rhiannon ; Woodcroft, Ben J. ; Kim, Eun Hae ; Mccalley, Carmody K. ; Hodgkins, Suzanne B. ; Crill, Patrick M. ; Chanton, Jeffrey ; Hurst, Gregory B. ; Verberkmoes, Nathan C. ; Saleska, Scott ; Hugenholtz, Philip ; Rich, Virginia I ; Tyson, Gene W. / Discovery of a novel methanogen prevalent in thawing permafrost. In: Nature Communications. 2014 ; Vol. 5.
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