Disruption of the fibroblast growth factor-2 gene results in decreased bone mass and bone formation

Aldemar Montero, Yosuke Okada, Masaro Tomita, Masako Ito, Hiroshi Tsurukami, Toshitaka Nakamura, Thomas Doetschman, J. Douglas Coffin, Marja M. Hurley

Research output: Contribution to journalArticle

353 Scopus citations

Abstract

Basic fibroblast growth factor (FGF-2), an important modulator of cartilage and bone growth and differentiation, is expressed and regulated in osteoblastic cells. To investigate the role of FGF-2 in bone, we examined mice with a disruption of the Fgf2 gene. Measurement of trabecular bone architecture of the femoral metaphysis of Fgf2(+/+) and Fgf2(-/-) adult mice by micro-CT revealed that the plate-like trabecular structures were markedly reduced and many of the connecting rods of trabecular bone were lost in the Fgf2(-/-) mice. Dynamic histomorphometry confirmed a significant decrease in trabecular bone volume, mineral apposition, and bone formation rates. In addition, there was a profound decreased mineralization of bone marrow stromal cultures from Fgf2(-/-) mice. This study provides strong evidence that FGF-2 helps determine bone mass as well as bone formation.

Original languageEnglish (US)
Pages (from-to)1085-1093
Number of pages9
JournalJournal of Clinical Investigation
Volume105
Issue number8
DOIs
StatePublished - Apr 2000
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

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    Montero, A., Okada, Y., Tomita, M., Ito, M., Tsurukami, H., Nakamura, T., Doetschman, T., Coffin, J. D., & Hurley, M. M. (2000). Disruption of the fibroblast growth factor-2 gene results in decreased bone mass and bone formation. Journal of Clinical Investigation, 105(8), 1085-1093. https://doi.org/10.1172/JCI8641