Disruption of the fibroblast growth factor-2 gene results in decreased bone mass and bone formation

Aldemar Montero, Yosuke Okada, Masaro Tomita, Masako Ito, Hiroshi Tsurukami, Toshitaka Nakamura, Thomas C Doetschman, J. Douglas Coffin, Marja M. Hurley

Research output: Contribution to journalArticle

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Abstract

Basic fibroblast growth factor (FGF-2), an important modulator of cartilage and bone growth and differentiation, is expressed and regulated in osteoblastic cells. To investigate the role of FGF-2 in bone, we examined mice with a disruption of the Fgf2 gene. Measurement of trabecular bone architecture of the femoral metaphysis of Fgf2(+/+) and Fgf2(-/-) adult mice by micro-CT revealed that the plate-like trabecular structures were markedly reduced and many of the connecting rods of trabecular bone were lost in the Fgf2(-/-) mice. Dynamic histomorphometry confirmed a significant decrease in trabecular bone volume, mineral apposition, and bone formation rates. In addition, there was a profound decreased mineralization of bone marrow stromal cultures from Fgf2(-/-) mice. This study provides strong evidence that FGF-2 helps determine bone mass as well as bone formation.

Original languageEnglish (US)
Pages (from-to)1085-1093
Number of pages9
JournalJournal of Clinical Investigation
Volume105
Issue number8
StatePublished - Apr 2000
Externally publishedYes

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Fibroblast Growth Factor 2
Osteogenesis
Bone and Bones
Genes
Bone Development
Thigh
Cartilage
Minerals
Bone Marrow
Cancellous Bone

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Montero, A., Okada, Y., Tomita, M., Ito, M., Tsurukami, H., Nakamura, T., ... Hurley, M. M. (2000). Disruption of the fibroblast growth factor-2 gene results in decreased bone mass and bone formation. Journal of Clinical Investigation, 105(8), 1085-1093.

Disruption of the fibroblast growth factor-2 gene results in decreased bone mass and bone formation. / Montero, Aldemar; Okada, Yosuke; Tomita, Masaro; Ito, Masako; Tsurukami, Hiroshi; Nakamura, Toshitaka; Doetschman, Thomas C; Coffin, J. Douglas; Hurley, Marja M.

In: Journal of Clinical Investigation, Vol. 105, No. 8, 04.2000, p. 1085-1093.

Research output: Contribution to journalArticle

Montero, A, Okada, Y, Tomita, M, Ito, M, Tsurukami, H, Nakamura, T, Doetschman, TC, Coffin, JD & Hurley, MM 2000, 'Disruption of the fibroblast growth factor-2 gene results in decreased bone mass and bone formation', Journal of Clinical Investigation, vol. 105, no. 8, pp. 1085-1093.
Montero A, Okada Y, Tomita M, Ito M, Tsurukami H, Nakamura T et al. Disruption of the fibroblast growth factor-2 gene results in decreased bone mass and bone formation. Journal of Clinical Investigation. 2000 Apr;105(8):1085-1093.
Montero, Aldemar ; Okada, Yosuke ; Tomita, Masaro ; Ito, Masako ; Tsurukami, Hiroshi ; Nakamura, Toshitaka ; Doetschman, Thomas C ; Coffin, J. Douglas ; Hurley, Marja M. / Disruption of the fibroblast growth factor-2 gene results in decreased bone mass and bone formation. In: Journal of Clinical Investigation. 2000 ; Vol. 105, No. 8. pp. 1085-1093.
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