Dissecting quantitative resistance against blast disease using heterogeneous inbred family lines in rice

Yan Liu, Gaisheng Zhang, Marichu Bernardo, Hei Leung, Xiao Yuan Zhu, Shaohong Zhang, Bin Liu, Jeremy Edwards, David W Galbraith, Jan Leach

Research output: Contribution to journalArticle

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Abstract

SHZ-2 is an indica rice cultivar that exhibits broad-spectrum resistance to rice blast; it is widely used as a resistance donor in breeding programs. To dissect the QTL responsible for broad-spectrum blast resistance, we crossed SHZ-2 to TXZ-13, a blast susceptible indica variety, to produce 244 BC4F3 lines. These lines were evaluated for blast resistance in greenhouse and Weld conditions. Chromosomal introgressions from SHZ-2 into the TXZ-13 genome were identiWed using a single feature polymorphism microarray, SSR markers and gene-speciWc primers. Segregation analysis of the BC4F3 population indicated that three regions on chromosomes 2, 6, and 9, designated as qBR2.1, qBR6.1, and qBR9.1, respectively, was associated with blast resistance and contributed 16.2, 14.9, and 22.3%, respectively, to the phenotypic variance of diseased leaf area (DLA). We further narrowed the three QTL regions using pairs of sister lines extracted from heterogeneous inbred families (HIF). Pairwise comparison of these lines enabled the determination of the relative contributions of individual QTL. The qBR9.1 conferred strong resistance, whereas qBR2.1 or qBR6.1 individually did not reduce disease under Weld conditions. However, when qBR2.1 and qBR6.1 were combined, they reduced disease by 19.5%, suggesting that small eVect QTLs contribute to reduction of epidemics. The qBR6.1 and qBR9.1 regions contain nucleotide-binding sites and leucine rich repeats (NBS-LRR) sequences, whereas the qBR2.1 did not. In the qBR6.1 region, the patterns of expression of adjacent NBS-LRR genes were consistent in backcross generations and correlated with blast resistance, supporting the hypothesis that multiple resistance genes within a QTL region can contribute to non-race-speciWc quantitative resistance.

Original languageEnglish (US)
Pages (from-to)341-353
Number of pages13
JournalTheoretical And Applied Genetics
Volume122
Issue number2
DOIs
StatePublished - Feb 2011

Fingerprint

blast disease
quantitative trait loci
rice
Leucine
Nucleotides
Binding Sites
Genes
Chromosomes, Human, Pair 9
Chromosomes, Human, Pair 6
Chromosomes, Human, Pair 2
leucine
binding sites
nucleotides
Breeding
Genome
genes
phenotypic variation
introgression
leaf area
Population

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Biotechnology
  • Medicine(all)

Cite this

Dissecting quantitative resistance against blast disease using heterogeneous inbred family lines in rice. / Liu, Yan; Zhang, Gaisheng; Bernardo, Marichu; Leung, Hei; Zhu, Xiao Yuan; Zhang, Shaohong; Liu, Bin; Edwards, Jeremy; Galbraith, David W; Leach, Jan.

In: Theoretical And Applied Genetics, Vol. 122, No. 2, 02.2011, p. 341-353.

Research output: Contribution to journalArticle

Liu, Y, Zhang, G, Bernardo, M, Leung, H, Zhu, XY, Zhang, S, Liu, B, Edwards, J, Galbraith, DW & Leach, J 2011, 'Dissecting quantitative resistance against blast disease using heterogeneous inbred family lines in rice', Theoretical And Applied Genetics, vol. 122, no. 2, pp. 341-353. https://doi.org/10.1007/s00122-010-1450-2
Liu, Yan ; Zhang, Gaisheng ; Bernardo, Marichu ; Leung, Hei ; Zhu, Xiao Yuan ; Zhang, Shaohong ; Liu, Bin ; Edwards, Jeremy ; Galbraith, David W ; Leach, Jan. / Dissecting quantitative resistance against blast disease using heterogeneous inbred family lines in rice. In: Theoretical And Applied Genetics. 2011 ; Vol. 122, No. 2. pp. 341-353.
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abstract = "SHZ-2 is an indica rice cultivar that exhibits broad-spectrum resistance to rice blast; it is widely used as a resistance donor in breeding programs. To dissect the QTL responsible for broad-spectrum blast resistance, we crossed SHZ-2 to TXZ-13, a blast susceptible indica variety, to produce 244 BC4F3 lines. These lines were evaluated for blast resistance in greenhouse and Weld conditions. Chromosomal introgressions from SHZ-2 into the TXZ-13 genome were identiWed using a single feature polymorphism microarray, SSR markers and gene-speciWc primers. Segregation analysis of the BC4F3 population indicated that three regions on chromosomes 2, 6, and 9, designated as qBR2.1, qBR6.1, and qBR9.1, respectively, was associated with blast resistance and contributed 16.2, 14.9, and 22.3{\%}, respectively, to the phenotypic variance of diseased leaf area (DLA). We further narrowed the three QTL regions using pairs of sister lines extracted from heterogeneous inbred families (HIF). Pairwise comparison of these lines enabled the determination of the relative contributions of individual QTL. The qBR9.1 conferred strong resistance, whereas qBR2.1 or qBR6.1 individually did not reduce disease under Weld conditions. However, when qBR2.1 and qBR6.1 were combined, they reduced disease by 19.5{\%}, suggesting that small eVect QTLs contribute to reduction of epidemics. The qBR6.1 and qBR9.1 regions contain nucleotide-binding sites and leucine rich repeats (NBS-LRR) sequences, whereas the qBR2.1 did not. In the qBR6.1 region, the patterns of expression of adjacent NBS-LRR genes were consistent in backcross generations and correlated with blast resistance, supporting the hypothesis that multiple resistance genes within a QTL region can contribute to non-race-speciWc quantitative resistance.",
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