Dissociation of brain tissue into viable single neurons in a microfluidic device

Linan Jiang, Robert Kraft, Linda L Restifo, Yitshak Zohar

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

A microfluidic technology-based tissue-dissociation device has for the first time been designed, fabricated and characterized for the purpose of primary neuronal cell culture. The system has been utilized for controlled dissociation, under an oscillatory flow field, of freshly explanted, enzyme-treated Drosophila larval central nervous system (CNS) into individual, viable neurons capable of robust outgrowth during in vitro culture. Device dimensions, constriction height and width, and operating conditions, flow-rate amplitude and frequency, have been determined based on video microscopy as well as quantitative analyses of the subsequent neuron-culture results.

Original languageEnglish (US)
Title of host publication9th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2015
PublisherIEEE Computer Society
Pages29-32
Number of pages4
Volume2016-June
ISBN (Electronic)9781467396714
DOIs
StatePublished - Jun 15 2016
Event9th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2015 - Honolulu, United States
Duration: Nov 15 2015Nov 18 2015

Other

Other9th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2015
CountryUnited States
CityHonolulu
Period11/15/1511/18/15

Fingerprint

Lab-On-A-Chip Devices
Microfluidics
Neurons
Brain
Tissue
Video Microscopy
Equipment and Supplies
Primary Cell Culture
Neurology
Cell culture
Constriction
Drosophila
Flow fields
Microscopic examination
Central Nervous System
Enzymes
Flow rate
Technology

Keywords

  • brain tissue dissociation
  • microfluidic device
  • neuron culture
  • oscillating flow field
  • single neurons

ASJC Scopus subject areas

  • Molecular Medicine
  • Biomedical Engineering
  • Microbiology (medical)

Cite this

Jiang, L., Kraft, R., Restifo, L. L., & Zohar, Y. (2016). Dissociation of brain tissue into viable single neurons in a microfluidic device. In 9th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2015 (Vol. 2016-June, pp. 29-32). [7492500] IEEE Computer Society. https://doi.org/10.1109/NANOMED.2015.7492500

Dissociation of brain tissue into viable single neurons in a microfluidic device. / Jiang, Linan; Kraft, Robert; Restifo, Linda L; Zohar, Yitshak.

9th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2015. Vol. 2016-June IEEE Computer Society, 2016. p. 29-32 7492500.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Jiang, L, Kraft, R, Restifo, LL & Zohar, Y 2016, Dissociation of brain tissue into viable single neurons in a microfluidic device. in 9th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2015. vol. 2016-June, 7492500, IEEE Computer Society, pp. 29-32, 9th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2015, Honolulu, United States, 11/15/15. https://doi.org/10.1109/NANOMED.2015.7492500
Jiang L, Kraft R, Restifo LL, Zohar Y. Dissociation of brain tissue into viable single neurons in a microfluidic device. In 9th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2015. Vol. 2016-June. IEEE Computer Society. 2016. p. 29-32. 7492500 https://doi.org/10.1109/NANOMED.2015.7492500
Jiang, Linan ; Kraft, Robert ; Restifo, Linda L ; Zohar, Yitshak. / Dissociation of brain tissue into viable single neurons in a microfluidic device. 9th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2015. Vol. 2016-June IEEE Computer Society, 2016. pp. 29-32
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