Dissociation of brain tissue into viable single neurons in a microfluidic device

Linan Jiang, Robert Kraft, Linda L. Restifo, Yitshak Zohar

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Scopus citations

Abstract

A microfluidic technology-based tissue-dissociation device has for the first time been designed, fabricated and characterized for the purpose of primary neuronal cell culture. The system has been utilized for controlled dissociation, under an oscillatory flow field, of freshly explanted, enzyme-treated Drosophila larval central nervous system (CNS) into individual, viable neurons capable of robust outgrowth during in vitro culture. Device dimensions, constriction height and width, and operating conditions, flow-rate amplitude and frequency, have been determined based on video microscopy as well as quantitative analyses of the subsequent neuron-culture results.

Original languageEnglish (US)
Title of host publication9th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2015
PublisherIEEE Computer Society
Pages29-32
Number of pages4
ISBN (Electronic)9781467396714
DOIs
StatePublished - Jun 15 2016
Event9th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2015 - Honolulu, United States
Duration: Nov 15 2015Nov 18 2015

Publication series

NameIEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED
Volume2016-June
ISSN (Print)2159-6964
ISSN (Electronic)2159-6972

Other

Other9th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2015
CountryUnited States
CityHonolulu
Period11/15/1511/18/15

Keywords

  • brain tissue dissociation
  • microfluidic device
  • neuron culture
  • oscillating flow field
  • single neurons

ASJC Scopus subject areas

  • Molecular Medicine
  • Biomedical Engineering
  • Microbiology (medical)

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