Distal hypoxic stroke: A new mouse model of stroke with high throughput, low variability and a quantifiable functional deficit

Kristian Doyle, Nancy Fathali, Mohammad R. Siddiqui, Marion S. Buckwalter

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

C57BL/6J are the most commonly used strain of mouse for stroke experiments but vascular anatomy of the Circle of Willis within this strain is extremely variable and the cortex has extensive collateralization. This causes large variability in stroke models that target the middle cerebral artery proximally and confers resistance to ischemia in those that target it distally. We tested the hypothesis that by combining distal middle cerebral artery occlusion with 1. h of hypoxia, we could generate a large lesion that causes a behavioral deficit with low variability. We found that this new distal hypoxic (DH) model of stroke generates a lesion with a volume of 25% of the ipsilateral hemisphere, extends to the motor cortex and causes a behavioral deficit. It also has a very clear border, exceptionally low variability, and can be performed by a single surgeon on up to 30 animals a day. Moreover, survivability is 100% in young adult animals, the model can be performed on old animals, and therapeutic intervention can reduce infarct volume. Therefore DH stroke is an excellent complement to existing stroke models and could be used for preclinical studies in C57BL/6J mice.

Original languageEnglish (US)
Pages (from-to)31-40
Number of pages10
JournalJournal of Neuroscience Methods
Volume207
Issue number1
DOIs
StatePublished - May 30 2012
Externally publishedYes

Fingerprint

Stroke
Circle of Willis
Middle Cerebral Artery Infarction
Middle Cerebral Artery
Motor Cortex
Inbred C57BL Mouse
Blood Vessels
Young Adult
Anatomy
Ischemia
Animal Models
Therapeutics

Keywords

  • Cerebral ischemia
  • Circle of Willis variability
  • Hypoxia
  • New mouse stroke model

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Distal hypoxic stroke : A new mouse model of stroke with high throughput, low variability and a quantifiable functional deficit. / Doyle, Kristian; Fathali, Nancy; Siddiqui, Mohammad R.; Buckwalter, Marion S.

In: Journal of Neuroscience Methods, Vol. 207, No. 1, 30.05.2012, p. 31-40.

Research output: Contribution to journalArticle

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