Distinct short-range ovule signals attract or repel Arabidopsis thaliana pollen tubes in vitro

Ravishankar Palanivelu, Daphne Preuss

Research output: Contribution to journalArticle

147 Citations (Scopus)

Abstract

Background: Pollen tubes deliver sperm after navigating through flower tissues in response to attractive and repulsive cues. Genetic analyses in maize and Arabidopsis thaliana and cell ablation studies in Torenia fournieri have shown that the female gametophyte (the 7-celled haploid embryo sac within an ovule) and surrounding diploid tissues are essential for guiding pollen tubes to ovules. The variety and inaccessibility of these cells and tissues has made it challenging to characterize the sources of guidance signals and the dynamic responses they elicit in the pollen tubes. Results: Here we developed an in vitro assay to study pollen tube guidance to excised A. thaliana ovules. Using this assay we discerned the temporal and spatial regulation and species-specificity of late stage guidance signals and characterized the dynamics of pollen tube responses. We established that unfertilized A. thaliana ovules emit diffusible, developmentally regulated, species-specific attractants, and demonstrated that ovules penetrated by pollen tubes rapidly release diffusible pollen tube repellents. Conclusion: These results demonstrate that in vitro pollen tube guidance to excised A. thaliana ovules efficiently recapitulates much of in vivo pollen tube behaviour during the final stages of pollen tube growth. This assay will aid in confirming the roles of candidate guidance molecules, exploring the phenotypes of A. thaliana pollen tube guidance mutants and characterizing interspecies pollination interactions.

Original languageEnglish (US)
Article number7
JournalBMC Plant Biology
Volume6
DOIs
StatePublished - Apr 5 2006
Externally publishedYes

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Pollen Tube
Ovule
pollen tubes
Arabidopsis
ovules
Arabidopsis thaliana
assays
In Vitro Techniques
Torenia fournieri
Species Specificity
Pollination
embryo sac
Haploidy
attractants
repellents
Diploidy
haploidy
Zea mays
Cues
Spermatozoa

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Distinct short-range ovule signals attract or repel Arabidopsis thaliana pollen tubes in vitro. / Palanivelu, Ravishankar; Preuss, Daphne.

In: BMC Plant Biology, Vol. 6, 7, 05.04.2006.

Research output: Contribution to journalArticle

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